Article

Analysis of the Major Patterns of B Cell Gene Expression Changes in Response to Short-Term Stimulation with 33 Single Ligands

University of California, San Diego, San Diego, California, United States
The Journal of Immunology (Impact Factor: 4.92). 01/2005; 173(12):7141-9. DOI: 10.4049/jimmunol.173.12.7141
Source: PubMed

ABSTRACT

We examined the major patterns of changes in gene expression in mouse splenic B cells in response to stimulation with 33 single ligands for 0.5, 1, 2, and 4 h. We found that ligands known to directly induce or costimulate proliferation, namely, anti-IgM (anti-Ig), anti-CD40 (CD40L), LPS, and, to a lesser extent, IL-4 and CpG-oligodeoxynucleotide (CpG), induced significant expression changes in a large number of genes. The remaining 28 single ligands produced changes in relatively few genes, even though they elicited measurable elevations in intracellular Ca(2+) and cAMP concentration and/or protein phosphorylation, including cytokines, chemokines, and other ligands that interact with G protein-coupled receptors. A detailed comparison of gene expression responses to anti-Ig, CD40L, LPS, IL-4, and CpG indicates that while many genes had similar temporal patterns of change in expression in response to these ligands, subsets of genes showed unique expression patterns in response to IL-4, anti-Ig, and CD40L.

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Available from: Jamie Lee, May 07, 2014
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    • "In addition Ca2+, phosphoinositide 3 kinase (PI3K), Erk1/2, canonical NF-кB, JNK1/2, p38a signalling can be initiated by B cell receptor activation [2,13-16]. In addition, aberrant signalling caused by a defined set of mutations or autocrine and paracrine loops for these pathways have been reported to be important for B cell lymphoma initiation or maintenance [2,11,17-19]. "
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