Abundance of IFN-? and IFN-? mRNA in blood of resistant and susceptible chickens infected with Marek?s disease virus (MDV) or vaccinated with turkey herpesvirus; and MDV inhibition of subsequent induction of IFN gene transcription

Institut National de la Recherche Agronomique, Bioagresseurs, Santé et Environnement, 37380 Nouzilly, France.
Archives of Virology (Impact Factor: 2.39). 04/2005; 150(3):507-19. DOI: 10.1007/s00705-004-0435-3
Source: PubMed


The effects of the very virulent RB-1B strain of Marek's disease virus (MDV) and turkey herpesvirus (HVT), a vaccinal strain, on abundance of IFN mRNA in the blood were investigated. MDV and HVT infection did not change the circulating level of IFN-gamma mRNA 1 and 7 days p.i., but they increased IFN-alpha mRNA levels slightly in genetically susceptible (to tumour development) B(13)/B(13) chickens. The total number of circulating leukocytes was unchanged and increase in message was accompanied by an increase in circulating CD8alpha(+) and MHC Class II(+) cells. On the contrary, both viruses slightly increased IFN-gamma transcripts and decreased IFN-alpha transcripts in genetically resistant B(21)/B(21) chickens. Further, oncogenic MDV was able to block the response to inactivated Newcastle disease virus, a potent inducer of IFN, in both chicken lines. The inhibiting effect on transcription was present for both IFN at days 1 and 7 p.i. in susceptible B(13)/B(13) chickens, but only at day 7 p.i. in resistant B(21)/B(21) chickens. By contrast, non-oncogenic HVT did not interfere with induction of either message at one day p.i. and MDV had a more suppressive effect than HVT on IFN gene transcription 7 days p.i. in B(21)/B(21) chickens. Thus, the strong ability of MDV to block induction of IFN gene transcription detected in the blood as soon as one day after infection in susceptible chickens, as opposed to resistant chickens, not only causes immunosuppression but also may be related to the virus's oncogenicity.

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Available from: Renata Novak Kujundžić, Jul 18, 2014
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    • "Previous studies showed that expression of many proinflammatory cytokine genes, including IFN-í µí»¼, IFN-í µí»¾, iNOS, IL-1í µí»½, IL-6 and IL-18, have been enhanced in chicken following infection with MDV [22] [23] [24] [25]. Additionally, the changes of these cytokines expression in vivo were influenced by genetic background of chicken and virulence of MDV [26] [27] [28]. Meanwhile, the expression of TLR-3 and TLR-7 genes was induced in the lungs of chicken response to MDV infection [23]. "
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    ABSTRACT: It has been known that the chicken's resistance to disease was affected by chicken's genetic background. And RLR-mediated antiviral pathway plays an important role in detection of viral RNA. However, little is known about the interaction of genetic background with RLR-mediated antiviral pathway in chicken against MDV infection. In this study, we adopted economic line-AA broilers and native Erlang mountainous chickens for being infected with MDV. Upon infection with MDV, the expression of MDA-5 was upregulated in two-breed chickens at 4, 7, and 21 d.p.i. It is indicated that MDA-5 might be involved in detecting MDV in chicken. Interestingly, the expression of IRF-3 and IFN- β genes was decreased in spleen and thymus of broilers at 21 d.p.i, but it was upregulated in immune tissues of Erlang mountainous chickens. And the genome load of MDV in spleen of broiler is significantly higher than that in Erlang mountainous chickens. Meanwhile, we observed that the death of broiler mainly also occurred in this phase. Collectively, these present results demonstrated that the expression patters of IRF-3 and IFN- β genes in chicken against MDV infection might be affected by the genetic background which sequently influence the resistance of chicken response to MDV.
    Full-text · Article · Apr 2013
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    • "Type I interferons, such as IFN-α, are well known to play a role in clearing herpesvirus-infected cells (Mossman and Ashkar, 2005). In relation to MDV infection, the expression of IFN-α is influenced by the genetic background of chickens (Quere et al., 2005). In MD-resistant chickens, the expression of IFN-α has been shown to be downregulated (Xing and Schat, 2000a). "
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    ABSTRACT: The bursa of Fabricius serves as an important tissue in the process of Marek's disease virus (MDV) pathogenesis, since B cells of the bursa harbor the cytolytic phase of MDV replication cycle. In the present study, host responses associated with MDV infection in the bursa of Fabricius of chickens were investigated. The expression of MDV phosphoprotein (pp)38 antigen, MDV glycoprotein (gB) and MDV viral interleukin (vIL)-8 transcripts was at the highest at 4 days post-infection (d.p.i.) and then showed a declining trend. On the contrary, the expression of meq (MDV EcoRI Q) gene as well as the viral genome load increased gradually until day 14 post-infection. The changes in viral parameters were associated with significantly higher infiltration of macrophages and T cell subsets, particularly CD4+ T cells into the bursa of Fabricius. Of the genes examined, the expression of interferon (IFN)-alpha, IFN-gamma genes and inducible nitric oxide synthase (iNOS) was significantly up-regulated in response to MDV infection in the bursa of Fabricius. The results suggest a role for these cells and cytokines in MDV-induced responses in the bursa of Fabricius.
    Full-text · Article · Oct 2008 · Virology
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    • "T cells, particularly CD8+ T cells (Omar et al., 1998) may play a role in vaccine-induced immune response against MD. In agreement with this CD8+ T cell-mediated immune function, the number of circulating CD8+ T cells increases following vaccination with HVT (Quere et al., 2005). Similar to HVT, CVI988 could also induce the expansion of both T cell subsets in the spleen (Gimeno et al., 2004). "
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    ABSTRACT: In Marek's disease virus infection, feather follicle epithelium (FFE) constitutes the site of formation of infectious virus particles and virus shedding. The objective of this study was to characterize cellular and cytokine responses as indicators of cell-mediated immune response in FFE and associated feather pulp following immunization against Marek's disease. Analysis of feather tips collected between 4 and 28 days post-immunization (d.p.i.) from chickens vaccinated post-hatch with either CVI988/Rispens or herpesvirus of turkeys revealed that replication of these vaccine viruses started at 7d.p.i., peaked by 21d.p.i., and subsequently, showed a declining trend. This pattern of viral replication, which led to viral genome accumulation in feather tips, was associated with infiltration of T cell subsets particularly CD8+ T cells into the feather pulp area and the expression of cytokine genes such as interferon-gamma, which is an indication of elicitation of cell-mediated immune responses at the site of virus shedding.
    Full-text · Article · Aug 2008 · Veterinary Immunology and Immunopathology
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