Niyogi KK, Li XP, Rosenberg V, Jung HS. Is PsbS the site of non-photochemical quenching in photosynthesis? J Exp Bot 56: 375-382

Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA.
Journal of Experimental Botany (Impact Factor: 5.53). 02/2005; 56(411):375-82. DOI: 10.1093/jxb/eri056
Source: PubMed


The PsbS protein of photosystem II functions in the regulation of photosynthetic light harvesting. Along with a low thylakoid lumen pH and the presence of de-epoxidized xanthophylls, PsbS is necessary for photoprotective thermal dissipation (qE) of excess absorbed light energy in plants, measured as non-photochemical quenching of chlorophyll fluorescence. What is known about PsbS in relation to the hypothesis that this protein is the site of qE is reviewed here.

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    • "ess light energy , absorbed by PSII , is eliminated as heat . It is the main component of non photochemical quenching ( NPQ ) . Thermal dissipation in plants requires zeaxanthin , a carotenoid that is involved in the xanthophyll cycle , and the action of PsbS , a chlorophyll binding protein within PSII ( Bugos and Yamamoto 1996 ; Li et al . 2000 ; Niyogi et al . 2005 ) . Li and colleagues proposed that the thermal dissipation of excitation energy might protect PSII from photoinhibition by decreasing the rate of photodamage to PSII ( Li et al . 2002 ) . However , more recent studies , in which photodamage and repair were monitored separately in NPQ - defective mutants of Arabidopsis , revealed that d"
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    ABSTRACT: When photosynthetic organisms are exposed to abiotic stress, their photosynthetic activity is significantly depressed. In particular, photosystem II (PSII) in the photosynthetic machinery is readily inactivated under strong light and this phenomenon is referred to as photoinhibition of PSII. Other types of abiotic stress act synergistically with light stress to accelerate photoinhibition. Recent studies of photoinhibition have revealed that light stress damages PSII directly, whereas other abiotic stresses act exclusively to inhibit the repair of PSII after light-induced damage (photodamage). Such inhibition of repair is associated with suppression, by reactive oxygen species (ROS), of the synthesis of proteins de novo and, in particular, of the D1 protein, and also with the reduced efficiency of repair under stress conditions. Gene-technological improvements in the tolerance of photosynthetic organisms to various abiotic stresses have been achieved via protection of the repair system from ROS and, also, by enhancing the efficiency of repair via facilitation of the turnover of the D1 protein in PSII. In this review, we summarize the current status of research on photoinhibition as it relates to the effects of abiotic stress and we discuss successful strategies that enhance the activity of the repair machinery. In addition, we propose several potential methods for activating the repair system by gene-technological methods.
    Full-text · Article · Aug 2014 · Applied Microbiology and Biotechnology
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    • "The PsbS subunit of PSII is considered to be a crucial component in the regulation of the PSII photochemistry, because PsbS mutants are defective in non-photochemical quenching (Li et al. 2000). In contrast to photochemical quenching, which describes the de-excitation of PSII with concomitant electron transport, non-photochemical quenching describes the reduction of PSII fluorescence due to the production of heat (Niyogi et al. 2005). "
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    ABSTRACT: Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221-226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A "milder" procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501-1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants.
    Full-text · Article · Aug 2013 · Photosynthesis Research
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    • "The main aim of this paper is the identification of possible relationships between light stress and 1 H NMR signals variations. Particular attention will be given to changes in carotenoids and xanthophylls in order to relate them to the well-known mechanism of Non-Photochemical Quenching (NPQ) triggered in the presence of excessive light energy (Horton et al. 1996; Niyogi et al. 2005; Pascal et al. 2005). "
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    ABSTRACT: In highlight stress conditions, the mechanism of non-photochemical quenching (NPQ) of chlorophyll fluorescence is triggered at the chloroplast level. This process allows thermal quenching of the excessive excitation energy and it is strictly related to the efficiency of the xanthophyll cycle. Nowadays, the utilization of the nuclear magnetic resonance (NMR) spectroscopy provides a powerful complementary way for the identification and quantitative analysis of plant metabolites either in vivo or in tissue extracts. Seeing that the oxidative damage caused by light stress in plants and the consequent involvement of pigments are widely studied, NMR spectroscopy can be utilized to compare crude leaf extract at different levels of light stress, allowing an analysis of these compounds. In this paper, the identification of possible relationships between light stress and (1)H NMR signal variations is discussed. The analysis of the (1)H NMR (1D) spectra of two agronomic species (Spinacia oleracea and Beta vulgaris) exposed to different light intensities is presented. In particular, change in carotenoids and xanthophylls signals are analyzed.
    Full-text · Article · May 2013 · Photosynthesis Research
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