Article

An unusual fold for potassium channel blockers: NMR structure of three toxins from the scorpion Opisthacanthus madagascariensis

Architecture et Fonction des Macromolécules Biologiques, UMR 6098, CNRS et Universités d'Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
Biochemical Journal (Impact Factor: 4.4). 06/2005; 388(Pt 1):263-71. DOI: 10.1042/BJ20041705
Source: PubMed

ABSTRACT

The Om-toxins are short peptides (23-27 amino acids) purified from the venom of the scorpion Opisthacanthus madagascariensis. Their pharmacological targets are thought to be potassium channels. Like Csalpha/beta (cystine-stabilized alpha/beta) toxins, the Om-toxins alter the electrophysiological properties of these channels; however, they do not share any sequence similarity with other scorpion toxins. We herein demonstrate by electrophysiological experiments that Om-toxins decrease the amplitude of the K+ current of the rat channels Kv1.1 and Kv1.2, as well as human Kv1.3. We also determine the solution structure of three of the toxins by use of two-dimensional proton NMR techniques followed by distance geometry and molecular dynamics. The structures of these three peptides display an uncommon fold for ion-channel blockers, Csalpha/alpha (cystine-stabilized alpha-helix-loop-helix), i.e. two alpha-helices connected by a loop and stabilized by two disulphide bridges. We compare the structures obtained and the dipole moments resulting from the electrostatic anisotropy of these peptides with those of the only other toxin known to share the same fold, namely kappa-hefutoxin1.

Download full-text

Full-text

Available from: Hervé Darbon, Mar 25, 2015
  • Source
    • "From O. madagascariensis, short chain affecting ionic channels were described with molecular masses between 4 kDa and 5 kDa that act as potassium (K + ) channel blockers (Chagot et al., 2005; Yamaji et al., 2004). Camargos et al., described two potassium-channels toxins (KTxs) from the venom gland transcriptome of O. cayaporum, blocking this channels, both similar to the ones described previously in O. madagascarensis venom (Camargos et al., 2011a; Chagot et al., 2005). "
    [Show abstract] [Hide abstract]
    ABSTRACT: We report the first biochemical, biological, pharmacological and partial proteomic characterization studies of the Opisthancanthus elatus venom (Gervais, 1844) from Colombia. The Reverse Phase High-Performance Liquid Chromatography venom profile showed 28 main well-defined peaks, most eluting between 20 and 45minutes (18-30% of acetonitrile respectively). High-resolution mass analysis indicates the presence of 106 components ranging from 806.59742Da to 16849.4139Da. O. elatus venom showed hemolytic activity and hydrolyzed the specific substrate BapNa suggesting the presence of proteins with serine-protease activity. Collected RP-HPLC fractions eluting at 52.6, 55.5, 55.8, 56.2, and 63.9min (PLA2 region between 33-40 % of acetonitrile), showed hemolytic activity and hydrolyzed the synthetic substrate 4-nitro-3-octanoyloxy-benzoic acid, indicating the presence of compounds with phospholipases A2 activity. These RP-HPLC fractions, showed molecular masses values up to 13978.19546Da, corroborating the possible presence of the mentioned enzymes. Tryptic digestion and MS/MS analysis showed the presence of a phospholipase like fragment, similar to on described in other Opisthacanthus genus studies. No coagulant activity was observed. No larvicidal or antimicrobial activity was observed at concentrations evaluated. Lethal and toxic activity is expected at doses above 100mg/kg, no neurotoxic effects were detected at lower doses. In conclusion, O. elatus exhibits a venom with a predominant phospholipase A2 activity than thought; mammal's neurotoxic activity is expected above the 100mg/kg, which is very high compared to the venom from other neurotoxic scorpions.
    Full-text · Article · Oct 2015 · Acta tropica
  • Source
    • "Kv1.3 channels at 500 mM (Chagot et al., 2005). OcyC8 (k- KTx2.5) inhibits the K þ currents of the Kv1.1 and Kv1.4 channels showing IC 50 values of 217 and 71, respectively (Camargos et al., 2011). "
    [Show abstract] [Hide abstract]
    ABSTRACT: The number and types of venom components that affect ion-channel function are reviewed. These are the most important venom components responsible for human intoxication, deserving medical attention, often requiring the use of specific anti-venoms. Special emphasis is given to peptides that recognize Na(+)-, K(+)- and Ca(++)-channels of excitable cells. Knowledge generated by direct isolation of peptides from venom and components deduced from cloned genes, whose amino acid sequences are deposited into databanks are now adays in the order of 1.5 thousands, out of an estimate biodiversity closed to 300,000. Here the diversity of components is briefly reviewed with mention to specific references. Structural characteristic are discussed with examples taken from published work. The principal mechanisms of action of the three different types of peptides are also reviewed. Na(+)-channel specific venom components usually are modifier of the open and closing kinetic mechanisms of the ion-channels, whereas peptides affecting K(+)-channels are normally pore blocking agents. The Ryanodine Ca(++)-channel specific peptides are known for causing sub-conducting stages of the channels conductance and some were shown to be able to internalize penetrating inside the muscle cells.
    Full-text · Article · Jul 2013 · Toxicon
  • Source
    • "Scorpion KTxs were originally classified into three families named ␣, ␤ and ␥ [29], all of them have the above mentioned, highly conserved, ␣/␤ structural arrangement. Latter, scorpion KTxs presenting a different structural arrangement, with only two ␣-helices stabilized by two disulfide bonds, CS␣/␣, were described, and they were named ␬-KTxs [6] [28] [4]. Among the almost 200 scorpion KTxs described until now (for a complete list see http://www.uniprot.org/docs/scorpktx), "
    [Show abstract] [Hide abstract]
    ABSTRACT: Opisthacanthus cayaporum belongs to the Liochelidae family, and the scorpions from this genus occur in southern Africa, Central America and South America and, therefore, can be considered a true Gondwana heritage. In this communication, the isolation, primary structure characterization, and K(+)-channel blocking activity of new peptide from this scorpion venom are reported. OcyKTx2 is a 34 amino acid long peptide with four disulfide bridges and molecular mass of 3807Da. Electrophysiological assays conducted with pure OcyKTx2 showed that this toxin reversibly blocks Shaker B K(+)-channels with a Kd of 82nM, and presents an even better affinity towards hKv1.3, blocking it with a Kd of ∼ 18nM. OcyKTx2 shares high sequence identity with peptides belonging to subfamily 6 of alpha-KTxs that clustered very closely in the phylogenetic tree included here. Sequence comparison, chain length and number of disulfide bridges analysis classify OcyKTx2 into subfamily 6 of the α-KTx scorpion toxins (systematic name, α-KTx6.17).
    Full-text · Article · May 2013 · Peptides
Show more