Proteomic analysis of the salt tolerance mutant of wheat under salt stress. Yi Chuan Xue Bao

College of Life Science, Hebei Normal University, Shijiazhuang 050016, China.
Acta Genetica Sinica 01/2005; 31(12):1408-14.
Source: PubMed


Two dimensional electrophoresis was used to analyse the proteome of the salt-tolerant mutant of wheat (RH8706-49) and the salt-sensitive mutant of wheat (H8706-34) which had been treated by 1% NaCl for 72 hours. After being analysed by MALDI-TOF-MS and Mascot software, the qualitative and quantitative differences were identified between the two materials for five candidate proteins: H+-transporting two-sector ATPase, glutamine synthetase 2 precursor, putative 33 kD oxygen evolving protein of photosystem II and ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit. These five proteins are all belong to chloroplast proteins. They are likely to play a crucial role in keeping the function of the chloroplast and the whole cells when the plant was under salt-stress.

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    • "There is a diverse expression pattern of saltresponsive genes found in different plants (especially glycophytes and halophytes) under different salinity conditions (e.g., salt concentration and treatment time) (Nito et al. 2007). The expression pattern of genes in cereals indicates that most of the proteins related with photosynthesis in O. sativa (Abbasi and Komatsu 2004; Kim et al. 2005; Parker et al. 2006), T. durum (Caruso et al. 2008), and T. aestivum (Huo et al. 2004; Peng et al. 2009) and proteins involved in carbohydrate and energy metabolism in O. sativa (Abbasi and Komatsu 2004; Chitteti and Peng 2007; Dooki et al. 2006; Kim et al. 2005; Li et al. 2010; Nohzadeh et al. 2007; Parker et al. 2006; Ruan et al. 2011), T. aestivum (Peng et al. 2009; Wang et al. 2008a), H. vulgare (Rasoulnia et al. 2010; Witzel et al. 2010), Z. mays (Zörb et al. 2004, 2010), and S. bicolor (Kumar et al. 2011) are induced by salinity. Most of the salt-responsive proteins are involved in basic metabolic processes like photosynthesis , energy metabolism, ROS scavenging, and ion homeostasis which make halophytes highly efficient in photosynthetic and energy metabolism, ion exclusion/compartmentalization , compatible product synthesis, induction of antioxidative enzymes and hormones, as well as modification of cell structure. "

    Full-text · Chapter · Jan 2013
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    • "In this study, number of Rubisco subunits was unevenly expressed in chloroplasts of water-stressed seedlings (Table 2). Isoforms of Rubisco activase likely play an important role in stabilizing and controlling proteolysis (Schwartz et al. 1995) and in maintaining chloroplast functioning during drought stress (Huo et al. 2004). Regulation of Rubisco activity has yet unclear under water stress. "
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    ABSTRACT: The performance of control and water-stressed 10-d-old wheat seedlings was compared. During short-term water stress (irrigation was withheld for 9 d), rates of photosynthesis and transpiration, stomatal conductance, and relative water content decreased whereas the proline content increased. Chloroplast proteins were extracted from the leaves, separated by iso-electric focusing through two-dimensional electrophoresis, and stained with CBB R-250. Differentially expressed proteins were detected and analyzed with MALDI-TOF/TOF mass spectrometry. Under water stress, 9 proteins were up-regulated whereas 11 proteins were not affected. The ribulose-1,5-bisphospate carboxylase/oxygenase (Rubisco) small and large subunits, chloride carrier/channel family, and H+-ATPase were up-regulated by water stress whereas membrane-bound ATP synthase subunit b and cytochrome b6-f complex were down-regulated.
    Full-text · Article · Nov 2012 · Biologia Plantarum
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    • "The found drought stress up-regulates proteins in K86-161 cultivar such as protease inhibitor, RuBisCO activase isoform and ferredoxin are interesting. These proteins are likely to play a role in stabilizing, controlling proteolysis (Schwartz et al., 1995) and maintaining the function of the chloroplast during plant exposure to drought stress (Huo et al., 2004). When compared to control K86-161, the Protease inhibitor was only detected in stressed plant, thus this protein was induced by subsequent induction after stress treatment. "

    Preview · Article · Apr 2010 · American Journal of Biochemistry and Biotechnology
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