Analysis of differentially expressed transcripts from planthopper-infested wild rice (Oryza minuta)

School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
Plant Cell Reports (Impact Factor: 3.07). 05/2005; 24(1):59-67. DOI: 10.1007/s00299-004-0905-9
Source: PubMed


A subtracted library was constructed from planthopper-infested wild rice (Oryza minuta) by suppression subtractive hybridization in combination with mirror orientation selection. To screen the differentially expressed transcripts in the library, we applied a cDNA microarray containing 960 random clones in a reverse Northern blot analysis using cDNA probes prepared from the mRNAs of control and planthopper-infested samples. On the basis of the signal intensities and expression ratios obtained from experiments performed in triplicate, we selected 383 clones. The elevated expression levels and overall profiles over time were verified by Northern blot analysis. Although Southern blot analysis showed similar copy numbers of the screened genes in O. minuta and O. sativa, it also revealed that the expression profiles had a different pattern. Functional categorization placed the identified transcripts in the categories of subcellular localization, metabolism, and protein fate. The presence of these expressed sequence tags implies that resistance of O. minuta to insect infestation can be achieved not only by an elevated expression of defense-related genes but also by enhanced metabolic activities.

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Available from: Ji-Ung Jeung, Jan 14, 2014
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    • "This discovery suggests that OsTPS13 is not a BPH feedinginducible STPS and that Os04g27430 expression is induced by both BPH feeding and other stresses, such as MeJA and wounding. Another STPS gene (Os08g07100) that is reportedly induced by BPH (Cho et al. 2005) was not polymorphic between KD and IL308 at the expression level in the present study (Figure 7A). This gene was induced by BPH feeding in both rice strains. "
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    • "). Sap sacking insects cause a reduction in phenylpropanoid related transcripts. For example, in rice, brown planthopper feeding down-regulated several genes involved in phenylpropanoid biosynthesis and up-regulated a gene required for sesquiterpene synthesis (Cho et al. 2005 "
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