Applications of zeolite inorganic composites in biotechnology: Current state and perspectives

ArticleinApplied Microbiology and Biotechnology 67(3):306-11 · June 2005with9 Reads
DOI: 10.1007/s00253-004-1782-4 · Source: PubMed
The purpose of this short review is to introduce applications of inorganic composites, zeolites, in biotechnology. Although inorganic chemistry is generally considered distant from biotechnology, the two could be harmoniously integrated for biopolymer chromatography. New chromatographic carriers have been developed based on principles differing from those underlying conventional chromatography. Some can be used for the purification of proteins according to novel physicochemical principles, according to their isoelectric point (pI), molecular weight and shape. The amount of protein adsorbed is related to the pore size of the composites, which can recognize biomolecules with reference to these three parameters. Proteins adsorbed at their pI have been found to be desorbed at the pI by polyethylene glycol, but not by high ionic medium (NaCl), SDS, non-ionic detergents, ATP or urea. Therefore, inorganic composites synthesized in consideration of pore size and three-dimensional structure are suitable as new chromatographic carriers. Selective fractionation of biomaterials including proteins and nucleic acids should provide useful information regarding whether conjugated proteins in a precipitated state can be separated on net charge and whether cells can be directly fractionated in future.
    • "his low-cost process may lead to production of zeolite NaY to be economically able to compete with established commercial zeolite [16]. Zeolites could selectively adsorb biopolymers like protein, DNA, and RNA, and therefore could be used as chromatographic carriers for these molecules [17][18][19][20]. For these reasons, zeolites would be able to selectively adsorb microbial cells and could be used as cell separation carriers in their native state without surface modiications. "
    Full-text · Article · Jan 2016 · Ceramics International
    • "This situation indicates that hydrophobic interactions could be more important for the adsorption process above the pI. It was reported that proteins cannot bind to silicalite (which has no Al), where Al molecules in zeolites may play a role in adsorption and above the pI, although there was electrostatic repulsion, some zeolites such as H-USYs were found to efficiently adsorb biopolymers[47]. This case was explained by the carboxylate anions on the protein structure, which could be substitute for water coordinating with metal ions, and the negative charges on proteins might result in the substitution of water at the Lewis acid sites of Al. "
    [Show abstract] [Hide abstract] ABSTRACT: The adsorption of the bacterial-glucuronidase (GUS) enzyme, which is thought to be responsible for the production of reactive metabolites related to some diseases and cancer development, by clinoptilolite-rich mineral was investigated. Batch experiments were performed to analyze of the effects of the clinoptilolite amount and particle size, initial GUS concentration, shaking rate, pH and temperature on the adsorption equilibrium and kinetics. Adsorption equilibrium data were interpreted in terms of Langmuir and Freundlich isotherms; and they were well represented by the Langmuir isotherm model. The percentage of GUS removal by the clinoptilolite-rich mineral was changed in the range of 9.4–54.4% depending on its initial concentration. The kinetic data were analyzed using external film diffusion, intraparticle diffusion, pseudo-first-order and pseudo-second-order models and both external film and intraparti-cle diffusion appeared to be effective in GUS adsorption. Thermodynamic studies indicated that GUS adsorption is exothermic, physical and spontaneous at the temperatures investigated (288–310 K).
    Full-text · Article · Jan 2015
    Dilek KavakDilek KavakDilek Demirbüker kavak
    • "However, the high thermal and chemical resistance of these materials allows their use in several severe environments and operating conditions. Furthermore, the inherent nontoxicity of the zeolite coatings could constitute an interesting alternative to the chromium-based surface treatment if we extended their applicability to food, pharmaceutical or biomedical applications1112. Certainly, the characteristic that mostly enhances the potential use of the zeolite coatings is the possibility to incorporate specific molecules or ions in the nanoporous structure [13]. "
    [Show abstract] [Hide abstract] ABSTRACT: This work reports the results of an experiment study on anti-corrosion coatings obtained by the direct growth of zeolite Y films on aluminium substrates by an amine-modified hydrothermal synthesis. In particular, the effect of the TEA (triethanolamine) presence was evaluated in terms of coating performances by varying synthesis times and drying temperatures. The morphological analysis showed that the films were homogeneous and compact up to 120 μm in thickness. Peel tests confirmed the good adhesive properties of all kinds of investigated coatings. In the end, electrochemical tests, carried out in 3.5% NaCl and in Ca(OH)2 saturated solution, showed good barrier properties offered by this type of coating. Low drying temperature allowed to keep TEA entrapped in the zeolite structure so that it greatly influenced corrosion protection behaviour.
    Full-text · Article · Sep 2014
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