Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100

Key Laboratory for Genetic Improvement of Oil Crops, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, People's Republic of China.
Applied Microbiology and Biotechnology (Impact Factor: 3.34). 11/2005; 68(6):802-7. DOI: 10.1007/s00253-005-1938-x
Source: PubMed


Sclerotinia sclerotiorum causes serious yield losses in oilseed crops worldwide. Bacillus subtilis Tu-100 significantly reduced (P< or =0.05) the incidence of disease caused by S. sclerotiorum on oilseed rape at harvest in two trials conducted in fields artificially infested with this pathogen. Mean plant dry weight was significantly greater (P< or =0.05) and mean plant length was significantly greater (P< or =0.07) at the seven-true-leaf stage with the Tu-100 treatment than with the control. Mean seed yield per 120 plants at harvest was significantly greater (P< or =0.05) in the second field trial with treatments containing isolate Tu-100. B. subtilis Tu-100 also promoted the growth of hydroponically grown oilseed rape. Plants were approximately 15% greater in dry weight (P< or =0.0001) and 6% greater in length (P< or =0.0025) when grown in the presence of isolate Tu-100 in Hoagland's solution, compared with the noninoculated control. In gnotobiotic studies, the lacZ-tagged strain B. subtilis Tu-100(pUC18) was detected within all roots of oilseed rape. Isolate Tu-100 did not persist in the ectorhizosphere of oilseed rape. Populations of this isolate decreased from 8.5x10(8) colony-forming units (CFU) per seed to approximately 10(2) CFU in the plant ectorhizosphere within 30 days of sowing in autoclaved soil.

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    • "Isolate Tri-1 was stored long term in 20% glycerol at _ 70°C. S. sclerotiorum Ss-1 was isolated from a sclerotium formed on oilseed rape (Hu et al. 2005). Isolate Ss-1 was maintained on PDA at 20 to 22°C. "
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    ABSTRACT: Sclerotinia sclerotiorum causes serious yield losses on many crops throughout the world. A multicomponent treatment that consisted of the residual rice straw remaining after rice harvest and Trichoderma sp. Tri-1 (Tri-1) formulated with the oilseed rape seedcake fertilizer was used in field soil infested with S. sclerotiorum. This treatment resulted in oilseed rape seed yield that was significantly greater than the nontreated control or when the fungicide carbendizem was used in the presence of this pathogen in field trials. Yield data suggested that the rice straw, oilseed rape seedcake, and Tri-1 components of this treatment all contributed incrementally. Similar treatment results were obtained regarding reduction in disease incidence. Slight improvements in yield and disease incidence were detected when this multicomponent treatment was combined with a fungicide spray. Inhibition of sclerotial germination by this multicomponent treatment trended greater than the nontreated control at 90, 120, and 150 days in field studies but was not significantly different from this control. This multicomponent treatment resulted in increased yield relative to the nontreated control in the absence of pathogen in a greenhouse pot study, while the straw alone and the straw plus oilseed rape seedcake treatments did not; suggesting that Tri-1 was capable of promoting growth. Experiments reported here indicate that a treatment containing components of a rice-oilseed rape production system augmented with Tri-1 can control S. sclerotiorum on oilseed rape, be used in integrated strategies containing fungicide sprays for control of this pathogen, and promote plant growth.
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    • "The detached leaf assay was performed as described by Hu et al. (2005). Leaves from greenhouse-grown oilseed rape (B. "
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    ABSTRACT: Sclerotinia sclerotiorum causes serious yield losses in oilseed rape and other crops worldwide. Field trials were conducted at two locations to evaluate two formulations of oilseed rape seed containing the plant-growth promoting bacterium Bacillus megaterium A6 for suppression of this pathogen. Treatments containing strain A6 in pellet and in wrap formulations resulted in oilseed rape seed yields that were similar to the chemical control and significantly greater than the untreated seed control at both locations. Treatments containing A6 in pellet and wrap formulations also resulted in an incidence of disease caused by S. sclerotiorum that was similar to the chemical control. Both of these treatments significantly decreased disease incidence relative to the untreated seed control and to the respective formulated seed controls (that did not contain strain A6) at both locations. Strain A6 applied to oilseed rape seed in these two formulations promoted growth in greenhouse pot studies conducted with autoclaved soil. In two experiments, these treatments resulted in significant increases in mean shoot dry weight per pot and mean % total N per plant relative to their respective controls containing formulated oilseed rape seed without strain A6 and to the untreated seed control. Both formulations provided stable B. megaterium A6 (≥106 CFU) and seed germination (>85%) over a six month period at room temperature. Experiments reported here indicate the commercial potential of these formulations of B. megaterium A6 for suppression of S. sclerotiorum on oilseed rape.
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    • "Agricultural Culture Collection of China (Beijing, China) as ACCC 05745. Another strain, B. megaterium ACCC 10407 is a known phosphate-solubilizing bacterium (Zhong and Huang 2005 "
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    ABSTRACT: Sustainable methods with diminished impact on the environment need to be developed for the production of oilseed rape in China and other regions of the world. A biological fertilizer consisting of Bacillus megaterium A6 cultured on oilseed rape meal improved oilseed rape seed yield (P < 0.0001) relative to the nontreated control in 2 greenhouse pot experiments using natural soil. This treatment resulted in slightly greater yield than oilseed rape meal without strain A6 in 1 of 2 experiments, suggesting a role for strain A6 in improving yield. Strain A6 was capable of solubilizing phosphorus from rock phosphate in liquid culture and produced enzymes capable of mineralizing organic phosphorus (acid phosphatase, phytase) in liquid culture and in the biological fertilizer. The biologically based fertilizer, containing strain A6, improved plant phosphorus nutrition in greenhouse pot experiments resulting in significantly greater available phosphorus in natural soil and in significantly greater plant phosphorus content relative to the nontreated control. Seed yield and available phosphorus in natural soil were significantly greater with a synthetic chemical fertilizer treatment, reduced in phosphorus content, than the biological fertilizer treatment, but a treatment containing the biological fertilizer combined with the synthetic fertilizer provided the significantly greatest seed yield, available phosphorus in natural soil, and plant phosphorus content. These results suggest that the biological fertilizer was capable of improving oilseed rape seed yield, at least in part, through the phosphorus-solubilizing activity of B. megaterium A6.
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