Identification of genes expressed differentially in subcutaneous and visceral fat of cattle, pig, and mouse

Department of Food Production Science, Faculty of Agriculture, Shinshu University, Nagano-ken, Japan.
Physiological Genomics (Impact Factor: 2.37). 06/2005; 21(3):343-50. DOI: 10.1152/physiolgenomics.00184.2004
Source: PubMed


The factors that control fat deposition in adipose tissues are poorly understood. It is known that visceral adipose tissues display a range of biochemical properties that distinguish them from adipose tissues of subcutaneous origin. However, we have little information on gene expression, either in relation to fat deposition or on interspecies variation in fat deposition. The first step in this study was to identify genes expressed in fat depot of cattle using the differential display RT-PCR method. Among the transcripts identified as having differential expression in the two adipose tissues were cell division cycle 42 homolog (CDC42), prefoldin-5, decorin, phosphate carrier, 12S ribosomal RNA gene, and kelch repeat and BTB domain containing 2 (Kbtbd2). In subsequent experiments, we determined the expression levels of these latter genes in the pig and in mice fed either a control or high-fat diet to compare the regulation of fat accumulation in other animal species. The levels of CDC42 and decorin mRNA were found to be higher in visceral adipose tissue than in subcutaneous adipose tissue in cattle, pig, and mice. However, the other genes studied did not show consistent expression patterns between the two tissues in cattle, pigs, and mice. Interestingly, all genes were upregulated in subcutaneous and/or visceral adipose tissues of mice fed the high-fat diet compared with the control diet. The data presented here extend our understanding of gene expression in fat depots and provide further proof that the mechanisms of fat accumulation differ significantly between animal species.

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Available from: Sanggun Roh, Aug 13, 2014
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    • "In a differential display approach, six genes were identified with expression differences in bovine VAT and SAT. All of these differences were reproducible in swine and mice, indicating that depot-specific factors exist over species 62. Li et al. (2012a, b) 63, 64 comparatively analyzed whole genome gene expression and methylation in SAT from three different locations, four VAT depots and intermuscular fat in swine, respectively. "
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    • "Results of the current study suggest that differences in expression patterns across adipose depots were more prominent than relatively minor changes in diet compo - sition . miRNA have been reported to be tissue specific in other species , including pigs ( Hishikawa et al . , 2005 ) , mice ( Gao et al . , 2011 ; Lagos - Quintana et al . , 2002 ) , and humans ( Liang et al . , 2007 ) , indicating the complex role that these regulators play in differentiating metabolism among tissues . Expression of miR - 101 and miR - 16b were upregulated in PAT compared to SAT , yet miR - 2454 was downregulated 16 . 8 - and 9 . 0"
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    • "Adipose tissue is dynamic and known to play an important role in energy homeostasis. Different fat depots are known to display distinct metabolic characteristics, such as distinctive gene expression profiles, and thus likely have distinctive physiology [27,28]. Additionally, the function and regulation of adipose tissue may be affected by many other factors, such as diet [29], age [30] and stress [31]. "
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