Article

HDAC6 Regulates Hsp90 Acetylation and Chaperone-Dependent Activation of Glucocorticoid Receptor

Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina 27710, USA.
Molecular Cell (Impact Factor: 14.02). 06/2005; 18(5):601-7. DOI: 10.1016/j.molcel.2005.04.021
Source: PubMed

ABSTRACT

The molecular chaperone heat shock protein 90 (Hsp90) and its accessory cochaperones function by facilitating the structural maturation and complex assembly of client proteins, including steroid hormone receptors and selected kinases. By promoting the activity and stability of these signaling proteins, Hsp90 has emerged as a critical modulator in cell signaling. Here, we present evidence that Hsp90 chaperone activity is regulated by reversible acetylation and controlled by the deacetylase HDAC6. We show that HDAC6 functions as an Hsp90 deacetylase. Inactivation of HDAC6 leads to Hsp90 hyperacetylation, its dissociation from an essential cochaperone, p23, and a loss of chaperone activity. In HDAC6-deficient cells, Hsp90-dependent maturation of the glucocorticoid receptor (GR) is compromised, resulting in GR defective in ligand binding, nuclear translocation, and transcriptional activation. Our results identify Hsp90 as a target of HDAC6 and suggest reversible acetylation as a unique mechanism that regulates Hsp90 chaperone complex activity.

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    • "(n=5 per group, normalized by housekeeping gene GAPDH expression; Fig. 3E). Inhibition of HDAC6 can affect the glucocorticoid receptors and in turn, affect anti-inflammatory responses like COX-2 expression (Kovacs et al., 2005; Zhang et al., 2008). However, other studies have shown that flow-induced COX-2 expression is largely dependent on extracellular signal-regulated kinase and Protein kinase A pathways (Wadhwa et al., 2002a,b). "
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    ABSTRACT: Mechanosensation is crucial for cells to sense and respond to mechanical signals within their local environment. While adaptation allows a sensor to be conditioned by stimuli within the environment and enables its operation in a wide range of stimuli intensities, the mechanisms behind adaptation remain controversial in even the most extensively studied mechanosensor, bacterial mechanosensitive channels. Primary cilia are ubiquitous sensory organelles. They have emerged as mechanosensors across diverse tissues, including kidney, liver and the embryonic node, and deflect with mechanical stimuli. Here, we show that both mechanical and chemical stimuli can alter cilium stiffness. We found that exposure to flow stiffens the cilium, which deflects less in response to subsequent exposures to flow. We also found that through a process involving acetylation, the cell can biochemically regulate cilium stiffness. Finally, we show that this altered stiffness directly affects the responsiveness of the cell to mechanical signals. These results demonstrate a potential mechanism through which the cell can regulate its mechanosensing apparatus.
    Preview · Article · Nov 2015 · Biology Open
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    • "HDAC6 is a class IIb HDAC with a structure similar to HDAC9; however , HDAC6 primarily resides in the cytoplasm. Substrates of HDAC6 include α-tubulin and Hsp90, which become deacetylated when HDAC6 catalyzes the removal of acetyl groups [25] [30]. We investigated the level of HDAC6 expression in immune cells isolated from early-and late-disease lupus-prone mice due to the role of HDAC6 in cell development and regulation of gene transcription. "
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    ABSTRACT: Prior studies have shown that pan-HDAC inhibition can decrease disease in lupus mice; however, the mechanisms(s) remain to be elucidated. MRL/MpJ-Fas(lpr) (MRL/lpr) mice develop a lupus-like disease characterized by anti-dsDNA production, lymphoproliferation, and immune complex-mediated glomerulonephritis. Early- and late-disease (12 and 20weeks-of-age respectively) female MRL/lpr mice were compared to age-matched, healthy C57BL/6 mice for HDAC expression and activity in bone marrow (BM) B cells, splenic B and T cells, and glomerular cells. We found that HDAC6 was significantly overexpressed in B cells, splenic T cells and glomerular cells, whereas HDAC9 expression was significantly increased in splenic T cells, BM B cells and glomerular cells. Due to the overexpression of HDAC6, we tested whether treatment with a selective HDAC6 inhibitor (ACY-738) or a pan-HDAC inhibitor (TsA) would decrease HDAC activity. ACY-738 significantly reduced cytoplasmic HDAC activity whereas TsA significantly decreased both nuclear and cytoplasmic HDAC activity. In vitro studies in mesangial cells showed that ACY-738 increased α-tubulin and Hsp90 acetylation resulting in decreased nuclear activation of NF-κB. Treatment of pre-B cells with ACY-738 decreased the Bcl-2:Bax ratio leading to a pro-apoptotic environment. These results suggest that increased HDAC6 expression and activity contribute to SLE pathogenesis, and isoform-selective HDAC inhibitors may prove beneficial in the treatment of SLE by acetylating key signaling and transcription factors in inflammation and cell activation.
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    • "Therefore, both HDAC4 and HDAC5 may be involved in the regulation of posttranslational processing of HIF-1a by regulating the Hsp70/Hsp90 system, but it is likely that HDAC5 plays a more important role in facilitating HIF-1a folding in response to hypoxic stress. HDAC6 inhibtion was proposed to increase the acetylation level of Hsp90, thus impairing Hsp90[Kovacs et al., 2005;Fath et al., 2006]. However, romidepsin (FK228,depsipeptide), an HDAC inhibitor that has very weak repressive effect on HDAC6, effectively destabilizes HIF-1a, making it possible that it also destabilizes HIF-1a through inhibiting HDAC5 or HDAC4, leading to Hsp70 hyperacetylation [Wang et al., 2007;To et al., 2011]. "
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