Summary. Bronchial glands have been regarded as
modified salivary glands. It is well known that there no
previous reviews concerning the antigenic profile of the
bronchial wall. The aim of this study is a systematic
survey of the antigenic profile and to describe the
histology of normal human bronchial glands. Six
formalin-fixed, paraffin-embedded surgical specimens
were studied using a panel of 22 polyclonal and
monoclonal antibodies by the avidin-biotin-peroxidase
method. Bronchial glands disclosed a tubuloacinar
structure. The smallest ducts intercalated originated from
a cluster of secretory acini and converge to form an
excretory duct. No striated duct was observed. Acinar
united is composed by mucous, serous and mixed units.
Myoepithelial cells are found in relation to the
intercalated ducts and secretory acinis. Secretory cells of
bronchial glands reacted strongly with cytokeratin AE1
and moderately for CK7, CK18. Additionally, serous
acinar cells reacted with AE3, CK19, CK5/6/8/18,
CK8/18/19, and Leu7. Myoepithelial cells reacted
strongly with α-smooth muscle actin, CD10 and
CK34ßE12. Ductal system cells differed from acinar
secretory cells in expressing CK34ßE12 and HSP27. In
conclusion, the detailed knowledge of the
immunohistochemical reactivities of normal cell types of
normal human bronchial glands will prove useful in
studies of bronchial pathology, especially of neoplastic
Key words: Immunohistochemical, Bronchial glands
The trachea and bronchial tree is endowed with a
rich supply of submucosal mixed, serous and mucous
glands, identical to those of the upper airways. Bronchial
glands are an endodermal derivative with a very similar
histological structure to salivary glands (Marchevsky,
1994). Morphologically, the mature bronchial glands
closely resemble mixed salivary glands, although their
ductal system is not as structurally complex as that of the
salivary glands. Although the histological structure and
inmunohistochemical profile of salivary tissues (Geiger
et al., 1987; Morinaga et al., 1987; Burns et al., 1988;
Gugliotta et al., 1988; Gustafsson et al., 1988) is well
known no previous report has been found addressing
both topics in human bronchial glands. We considered
that the knowledge of the immunohistochemical profile
of normal cells types of human bronchial glands would
be useful in studies of bronchial pathology, especially to
understand the histogenesis of some bronchial neoplasias
as mucoepidermoid tumors.
The aim of the present work is to study the
expression of intermediate filaments as well as CD10
and S-100 proteins, in human normal bronchial glands.
Materials and methods
Six formalin-fixed, paraffin-embedded bronchial
samples obtained from surgical lobectomy specimens
were used. Glands were considered as normal when no
inflammation or hyperplasia were present. All cases
showed a Reid index below 0.25 mm in spite of the fact
that the specimens come from patients who smoked.
Sections 4 µm thick, were cut up and mounted on
glass slides. For immunohistochemical techniques the
specimens were deparaffinized in xylene and rehydrated
step by step with descending concentrations of ethanol.
The sections were incubated at 37°C with 0.3% H2O2in
absolute methanol for 10 min, to block endogenous
peroxidase. After washing with phosphate-buffered
saline (PBS), for pH 7.2 for 20 min. they were incubated
with primary antibodies for 45 min in a moist chamber at
room temperature. Primary antibodies used were: anti-S-
100 (Dako, diluted 1:1500), anti-desmin (Dako, pre-
diluted 1:2), anti-chromogranin A (Dako, pre-diluted
1:2), anti-synaptophysin (Biomeda, pre-diluted 1:5),
anti-Leu-7 (Becton Dickonson, pre-dilution 1:20), anti-
HSP27 (Biogenex, pre-dilution 1:2), anti-HSP70
(Biogenex pre-dilution 1:2), anti-α-smooth muscle actin
(Enzo, pre-dilution 1:2), anti-CD31 (Novocastra,
dilution 1:30), anti-CD34 (Biogenex, pre-dilution), anti-
34ßE12 (Dako, dilution 1:2), anti-AE1 (Biomeda, pre-
dilution 1:2), anti-AE3 (Biomeda, pre-dilution 1:2), anti-
CK7 (Novocastra, dilution 1:50), anti-CK8 (Novocastra,
Antigenic profile of human bronchial gland
N. Sánchez-Mora1, J. Rendón-Henao1, V. Monroy1, M. Herranz Aladro1and E. Álvarez-Fernández1
1Gregorio Marañón General University General Hospital, Madrid, Spain and
2La Raza National Medical Centre Speciality Hospital, DF, México
Histol Histopathol (2005) 20: 865-870
Offprint requests to: Nora Sánchez Mora, Hospital General Universitario
Gregorio Marañón, C/ Dr. Esquerdo Nº 46, Dpto. de Anatomía
Patológica, 28007 Madrid, Spain..e-mail: firstname.lastname@example.org
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Accepted April 9, 2005
Human bronchial gland