Experimental Actinobacillus pleuropneumoniae infection in piglets with different types and levels of specific protection: Immunophenotypic analysis of lymphocyte subsets in the circulation and respiratory mucosal lymphoid tissue

Department of Immunology, Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic.
Veterinary Immunology and Immunopathology (Impact Factor: 1.54). 09/2005; 107(1-2):143-52. DOI: 10.1016/j.vetimm.2005.04.007
Source: PubMed


Actinobacillus pleuropneumoniae (APP) infection in piglets results in severe and fatal fibrinous hemorrhagic necrotizing pneumoniae. The aim of our study was to analyze changes in lymphocyte subset distribution in peripheral blood, bronchoalveolar lavage fluid (BALF) and tracheobronchal lymph nodes (TLN) in non-immune piglets upon a challenge with a high dose of APP and to compare the quality of such changes in unprotected piglets with counterparts exhibiting specific immunity mediated by high titers of colostrum-derived APP-specific antibodies and/or a low dose APP infection in the early postnatal period. Challenge with APP resulted in a massive increase in CD8-negative gammadelta T-cells in parallel with a reduction in numbers of CD3-CD8low cells in BALF independent of the type and level of immunity and this seems to be a general phenomenon associated with experimental infection. An increase in B-lymphocyte numbers in TLN was another characteristic feature accompanying APP infection in all experimental groups. In piglets with colostrum-derived APP-specific antibodies, this was associated with higher relative numbers of IgM+CD2+ lymphocytes in TLN, while B-cells with the CD2- surface phenotype apparently expanded in the absence of passive humoral immunity.

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Available from: Miroslav Toman, Dec 03, 2014
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    • "One of the most striking findings was the apparent proportional predominance of CD8 À cd T lymphocytes recruited to the compromised uterine tissue. Although cd T lymphocytes represent only a small population in the peripheral blood and lymphoid organs of adult dogs (Faldyna et al., 2001b, 2005a), they can be plausibly recruited to sites of inflammation as described in piglets (Faldyna et al., 2005b), where cd T lymphocytes recruited to the bronchoalveolar space after experimental infection with Actinobacillus pleuropneumoniae are also predominantly CD8 À (Faldyna et al., 2005b). Based on evidence derived from swine, in which CD8 À cd T lymphocytes occur predominantly in blood, while CD8 + cd T lymphocytes are mainly found in tissue (Yang and Parkhouse, 1996), blood can represent a source of CD8 À cd T lymphocytes. "
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    • "Clinical signs and pathological changes of the disease already appear within a few hours after experimental infection [1]. The infection of non-immunized pigs is followed by destruction of alveolar macrophages and rapid influx of professional phagocytes and lymphocytes to the tissue [1] and bronchoalveolar space [7] [8]. A rapid cellular influx of MP into infected lungs together with a specific localization of the pathogen in the lungs predetermine experimental APP infection to be an appropriate model for observing MP migration under inflammatory conditions in pigs. "
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