H2B (Ser10) Phosphorylation is Induced during Apoptosis and Meiosis in S. cerevisiae

Cornell University, Итак, New York, United States
Cell cycle (Georgetown, Tex.) (Impact Factor: 4.57). 07/2005; 4(6):780-3. DOI: 10.4161/cc.4.6.1745
Source: PubMed


The nucleosome, composed of an octamer of highly conserved histone proteins and associated DNA, is the fundamental unit of eukaryotic chromatin. How arrays of nucleosomes are folded into higher-order structures, and how the dynamics of such compaction are regulated, are questions that remain largely unanswered. Our recent studies demonstrated that phosphorylation of histone H2B is necessary to induce cell death that exhibits phenotypic hallmarks of apoptosis including DNA fragmentation and chromatin condensation in yeast (serine 10)1 and in mammalian cells (serine 14)2. In this article, we extend these findings by uncovering a role for H2B phosphorylation at serine 10 (Ser10) in another biological event that is associated with dramatic alterations in higher-order chromatin structure, meiosis. Our data show strong staining, indicative of H2B (Ser10) phosphorylation, during the pachytene stage of yeast meiotic prophase. These data broaden the use of this phosphorylation mark in chromatin remodeling that closely correlates with chromatin compaction. How phosphorylation marks are translated into meaningful downstream events during processes as diverse as apoptosis and meiosis remain a challenge for future studies.

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    • "Chromatin changes: As cells progress through meiosis, different histone modifications appear (Ahn et al. 2005; Borde et al. 2009; Govin et al. 2010a). While some occur early and are likely linked to the chromosome pairing events of meiotic prophase, other modifications accumulate as cells undergo the meiotic divisions. "
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    ABSTRACT: In response to nitrogen starvation in the presence of a poor carbon source, diploid cells of the yeast Saccharomyces cerevisiae undergo meiosis and package the haploid nuclei produced in meiosis into spores. The formation of spores requires an unusual cell division event in which daughter cells are formed within the cytoplasm of the mother cell. This process involves the de novo generation of two different cellular structures: novel membrane compartments within the cell cytoplasm that give rise to the spore plasma membrane and an extensive spore wall that protects the spore from environmental insults. This article summarizes what is known about the molecular mechanisms controlling spore assembly with particular attention to how constitutive cellular functions are modified to create novel behaviors during this developmental process. Key regulatory points on the sporulation pathway are also discussed as well as the possible role of sporulation in the natural ecology of S. cerevisiae.
    Preview · Article · Nov 2011 · Genetics
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    • "In fact, in response to different stimuli S. cerevisiae commits to cell death showing typical hallmarks of metazoan apoptosis [4]. Although several yeast orthologues of key apoptotic regulators have already been identified [5] [6] [7] [8] [9] [10], how they work in yeast programmed cell death (PCD) remains to be fully established. "
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    ABSTRACT: Yeast cells lacking the metacaspase-encoding gene YCA1 (Deltayca1) were compared with wild-type (WT) cells with respect to the occurrence, nature and time course of acetic-acid triggered death. We show that Deltayca1 cells undergo programmed cell death (PCD) with a rate lower than that of the WT and that PCD in WT cells is caused at least in part by the caspase activity of Yca1p. Since in Deltayca1 cells this effect is lost, but z-VAD-fmk does not prevent both WT and Deltayca1 cell death, PCD in WT cells occurs via a Yca1p caspase and a non-caspase route with similar characteristics.
    Full-text · Article · Jan 2007 · FEBS Letters
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    • "mating leads to the generation of diploid cells, which can, as a consequence of scarce nutrition, undergo meiosis and sporulate as a way to stochastically reshuffl e and rearrange the genome to increase genetic diversity and, thus, the fi tness of the population. This meiosis is coupled to apoptosis, as 20% of cells grown on sporulation media undergo apoptotic cell death, whereas the 80% that survive initiate sporulation (Ahn et al., 2005b; Knorre et al., 2005). This might ensure that only genetic recombinants that are adapted to their surroundings survive. "
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    ABSTRACT: The purpose of apoptosis in multicellular organisms is obvious: single cells die for the benefit of the whole organism (for example, during tissue development or embryogenesis). Although apoptosis has also been shown in various microorganisms, the reason for this cell death program has remained unexplained. Recently published studies have now described yeast apoptosis during aging, mating, or exposure to killer toxins (Fabrizio, P., L. Battistella, R. Vardavas, C. Gattazzo, L.L. Liou, A. Diaspro, J.W. Dossen, E.B. Gralla, and V.D. Longo. 2004. J. Cell Biol. 166:1055-1067; Herker, E., H. Jungwirth, K.A. Lehmann, C. Maldener, K.U. Frohlich, S. Wissing, S. Buttner, M. Fehr, S. Sigrist, and F. Madeo. 2004. J. Cell Biol. 164:501-507, underscoring the evolutionary benefit of a cell suicide program in yeast and, thus, giving a unicellular organism causes to die for.
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