Article

Barberi T, Willis LM, Socci ND, Studer LDerivation of multipotent mesenchymal precursors from human embryonic stem cells. PLoS Med 2:e161

Memorial Sloan-Kettering Cancer Center, New York, New York, United States
PLoS Medicine (Impact Factor: 14.43). 07/2005; 2(6):e161. DOI: 10.1371/journal.pmed.0020161
Source: PubMed

ABSTRACT

Human embryonic stem cells provide access to the earliest stages of human development and may serve as a source of specialized cells for regenerative medicine. Thus, it becomes crucial to develop protocols for the directed differentiation of embryonic stem cells into tissue-restricted precursors.
Here, we present culture conditions for the derivation of unlimited numbers of pure mesenchymal precursors from human embryonic stem cells and demonstrate multilineage differentiation into fat, cartilage, bone, and skeletal muscle cells.
Our findings will help to elucidate the mechanism of mesoderm specification during embryonic stem cell differentiation and provide a platform to efficiently generate specialized human mesenchymal cell types for future clinical applications.

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Available from: Tiziano Barberi, Mar 20, 2015
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    • "GFP and dystrophinpositive fibers were detected in the muscles of only two out of eight mice transplated with cells, and one of these mice had as few as 30 of these fibers (Bhagavati and Xu 2005). Together, these results indicate that the co-culture system is not efficient in the induction of myogenic differentiation of PSCs (Barberi et al. 2005; Bhagavati and Xu 2005; Archacka et al. 2014). In addition, although the co-culture system yields important information about myogenic potential of stem cells and reciprocal interactions between co-cultured cells, this method is not likely to be clinically suitable. "
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    • "Barbet et al. identified differentially expressed genes utilising a Taqman low-density array and reported that hESC-MSC clustered more closely with BM-hMSC than with undifferentiated hESC (Barbet et al., 2011). Barberi et al. utilised affymetrix oligonucleotide arrays on a CD73 + sorted population derived from hESC and compared these with BM-hMSC (Barberi et al., 2005). Similar to our data the authors found enrichment for genes associated with vascular development and inflammatory response/wound healing. "
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    • "Several groups described strategies to derive MSC-like cells from either ESCs (Barberi et al., 2005; Boyd et al., 2009) or iPSCs (Liu et al., 2012; Diederichs and Tuan, 2014; Zhang et al., 2011). These approaches were based on coculture with primary MSCs, growth factor combinations , or spontaneous differentiation in embryoid bodies (EBs). "
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