[Study of Five STR Loci in A Chinese Han Population.].
School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. Hereditas (Beijing)
To understanding the allele structure and genetic polymorphisms at five STR loci in Chinese Han population, and construct a preliminary database, EDTA-blood specimens were collected from unrelated individuals. DNA samples were extracted with Chelex method and were amplified by PCR technique. The PCR products were analyzed using both PAGE horizontal electrophoresis with discontinuous buffer system and automated fluorescence detection approach. As a result, three STRs consist of simple repeat motifs, while two STR contain a complex repeat structure. The STR polymorphisms at all of the five loci have been observed in Chinese Han population. In a word, the obtained data are beneficial to understanding the population genetics of the five STR loci in Chinese Han population. As a simple approach, the PAGE horizontal electrophoresis can be employed for typing the five STR markers.
Available from: Zhaoshu Zeng
- "However, there are still some issues to be considered, which is necessity in order to i) obtain different population genetic information from different populations; ii) know the motif of core sequence for some STR loci because there were different motif repeats reported in different populations, such as D22GATA198B05 locus (Zhao et al., 2002; Jia et al., 2005; Zhang et al., 2006) ; iii) get detailed information of different motif repeats for the compound or complex STR loci. Due to allele structure or DNA sequence variation in the alleles of particular STR loci, sequence analysis of individual alleles is essential for the use of these markers for human identification. "
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ABSTRACT: This study was carried out to investigate the genetic polymorphism of nine short tandem repeat (STR) loci including D2S1772, D6S1043, D7S3048, D8S1132, D11S2368, D12S391, D13S325, D18S1364 and D22GATA198B05 in Chinese Han population of Henan province and to assess its value in forensic science. Genomic DNA was extracted by means of phenol-chloroform from 212 blood samples of unrelated healthy individuals in Henan Han population, and alleles were amplified with STRtyper-10F/G kit. The genotype distributions were in accordance with Hardy-Weinberg equilibrium (p>0.05). New core sequences were found in D2S1772, D7S3048, and D22GATA198B05 STR loci in Chinese Han population of Henan province, which were different from those of other populations. Two single-nucleotide polymorphism (SNP) sites were found at locus D18S1364 and D22GATA198B05, respectively. The heterozygosity (H), power of discrimination (PD), probability of exclusion (PE) and polymorphism information contents (PIC) ranged from 0.778 to 0.887, 0.928 to 0.967, 0.559 to 0.769 and 0.77 to 0.86, respectively. The total PD and combined PE of nine STR loci reached 0.999999999999363 and 0.999951, respectively. The nine STR loci are highly polymorphic in Chinese Han population of Henan province and they may be of great value in forensic science and human population genetics.
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