Sulforaphane prevents mouse skin tumorigenesis during the stage of promotion. Cancer Lett

University of Illinois, Urbana-Champaign, Urbana, Illinois, United States
Cancer Letters (Impact Factor: 5.62). 06/2006; 236(1):72-9. DOI: 10.1016/j.canlet.2005.05.007
Source: PubMed


Sulforaphane (SF), a natural product from broccoli, is known to enhance detoxification of carcinogens and block initiation of chemically-induced carcinogenesis in animal models. Cell culture and xenograft studies suggest additional roles for SF, inhibiting growth of tumors, arresting the cell cycle and enhancing apoptosis. As currently reported, topical SF (1, 5 or 10 micromol/mouse) significantly inhibited 7,12-dimethylbenz(a)anthracene/12-O-tetradecanoylphorbol 13-acetate (TPA)-induced mouse skin tumorigenesis, using either an anti-promotion protocol (SF from 1 week after carcinogen until the end of the study) or a combined anti-initiation, anti-promotion protocol (SF 7 days prior to carcinogen until the end of the study). Surprisingly, no significant effect was observed in an anti-initiation protocol (SF from 7 days prior to 7 days after carcinogen). Separately, SF inhibited TPA-induced ornithine decarboxylase activity in mouse skin, an obligate step in TPA-induced promotion of carcinogenesis. These data link this molecular mechanism to SF-dependent inhibition of the promotion of tumorigenesis.

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    • "Sulforaphane is formed from its glucosinolate (glucoraphanin) by myrosinase when broccoli tissue is crushed or chewed. More epidemiological studies have shown that sulforaphane has antitumor activity by enhancing detoxification of carcinogens and blocking the initiation of chemically induced carcinogenesis in animal models (Gills et al., 2006; Zhang, 2004). Broccoli is harvested when the flowering heads are immature (Tian et al., 1994). "
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    ABSTRACT: The effect of sucrose treatment on shelf life, the color, contents of glucosinolate, sulforaphane, total phenols, and antioxidant activity in broccoli florets was investigated. The results showed that sucrose (12gL-1) treatment extended shelf life, inhibited the increase of L* value, retained a high hue angle and chlorophyll content. The decreases of glucosinolate and sulforaphane contents in broccoli florets were suppressed after sucrose treatment. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), phenylalanine ammonia-lyase (PAL) and catalase (CAT) were enhanced in sucrose-treated florets, whereas the activity of peroxidase (POD) was significantly reduced. Sucrose treatment also enhanced total phenolic content and maintained higher levels of total and reducing sugars. These results indicated that sucrose treatment can prolong shelf life in non-refrigerated retail display cases, maintain quality and improve the nutritional value of postharvest broccoli florets.
    Full-text · Article · Feb 2016 · Postharvest Biology and Technology
    • "However, a very efficient tumor-suppressive effect of sulforaphane was observed when the application was started one week after DMBA treatment and continued until the end of the tumor observation. This finding suggests that sulforaphane is required during the stage of tumor promotion [60]. The efficacy of sulforaphane for cancer prevention was confirmed in in vitro studies with cultured JB6 keratinocytes, where sulforaphane inhibited TPA-induced cellular transformation, which was abrogated on Nrf2 knockdown. "
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    ABSTRACT: The skin is frequently exposed to environmental challenges, such as UV irradiation, toxic chemicals or mechanical wounding. These insults cause an increase in the levels of reactive oxygen species, resulting in oxidative stress and concomitant inflammation, skin aging and even cancer development. Therefore, an efficient antioxidant defense strategy is of major importance in this tissue. Since the NRF2 transcription factor regulates a battery of genes involved in the defense against reactive oxygen species and in compound metabolism, it plays a key role in skin homeostasis, repair and disease. In this review we summarize current knowledge on the expression and function of NRF2 in normal skin, its role in the acute and chronic UV response as well as in the pathogenesis of epithelial skin cancer and of different inflammatory skin diseases. Finally, we discuss the potential of NRF2-activating compounds for skin protection under stress conditions and for the treatment of major human skin disorders. Copyright © 2015. Published by Elsevier Inc.
    No preview · Article · Apr 2015 · Free Radical Biology and Medicine
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    • "Chemical proteomics aimed at characterizing the effects of drug candidates could sometimes lead to the discovery of new drug targets. For example, sulforaphane (SFN) is known to have antimicrobial and anticancer properties in experimental models.110–112 SFN can modulate multiple cellular targets involved in cancer development including DNA protection, inhibition of cancer cell proliferation, induction of apoptosis, inhibition of neoangiogenesis, progression of benign tumors to malignant tumors, and metastasis.112,113 "
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    ABSTRACT: Proteomic approaches are continuing to make headways in cancer research by helping to elucidate complex signaling networks that underlie tumorigenesis and disease progression. This review describes recent advances made in the proteomic discovery of drug targets for therapeutic development. A variety of technical and methodological advances are overviewed with a critical assessment of challenges and potentials. A number of potential drug targets, such as baculoviral inhibitor of apoptosis protein repeat-containing protein 6, macrophage inhibitory cytokine 1, phosphoglycerate mutase 1, prohibitin 1, fascin, and pyruvate kinase isozyme 2 were identified in the proteomic analysis of drug-resistant cancer cells, drug action, and differential disease state tissues. Future directions for proteomics-based target identification and validation to be more translation efficient are also discussed.
    Full-text · Article · Oct 2013 · Drug Design, Development and Therapy
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