Bioactive pectic polysaccharides from Glinus oppositifolius (L.) Aug. DC., a Malian medicinal plant, isolation and partial characterization

Article (PDF Available)inJournal of Ethnopharmacology 101(1-3):204-14 · November 2005with76 Reads
DOI: 10.1016/j.jep.2005.04.021 · Source: PubMed
Glinus oppositifolius (L.) Aug. DC. (Aizoaceae) is a Malian medicinal plant used against various types of illnesses related to the immune response, like joint pains, inflammations, fever, malaria and wounds. Two pectin type polysaccharides, GOA1 and GOA2, being isolated from a 50 degrees C water extract from the aerial parts of Glinus oppositifolius were investigated for their activity towards the complement system and different leukocyte subsets because of the assumed effects on conditions related to the immune system. The polysaccharide polymer in GOA1 was shown to contain considerable amounts of the neutral sugars arabinose (26.4 mol%) and galactose (42.9 mol%), and methylation analysis indicated the presence of arabinogalactans type I (AG-I) and type II (AG-II). GOA2 was rich in galacturonic acid (68.3 mol%), along with rhamnose, arabinose and galactose. Structural studies indicated that rhamnose and galacturonic acid might constitute a rhamnogalacturonan backbone, often found in pectic substances, with side chains consisting of arabinose and galactose. Both GOA1 and GOA2 were shown to exhibit potent dose-dependent complement fixating activities, and induced chemotaxis of macrophages, T cells and NK cells.
    • "The activation of innate immune cells and of the complement system can also be involved in the activation of other cells and adaptive immunity. Nevertheless, direct activation by immunostimulatory polysaccharides of other immune cells than macrophages, like NK cells and B lymphocytes was also shown (Inngjerdingen et al., 2005Inngjerdingen et al., , 2007a ). The polysaccharides' stimulatory effect on lymphocyte cells has been evaluated mainly by induction of proliferation (Du et al., 2014; Inngjerdingen et al., 2007a; Qiao et al., 2010b; Wang, Liu, & Fang, 2005; Zhao et al., 2010), and cytokine production (Holderness et al., 2011; Nosál'ová et al., 2011; Skyberg et al., 2012). "
    [Show abstract] [Hide abstract] ABSTRACT: Immunostimulatory polysaccharides are compounds capable of interacting with the immune system and enhance specific mechanisms of the host response. Glucans, mannans, pectic polysaccharides, arabinogalactans, fucoidans, galactans, hyaluronans, fructans, and xylans are polysaccharides with reported immunostimulatory activity. The structural features that have been related with such activity are the monosaccharide and glycosidic-linkage composition, conformation, molecular weight, functional groups, and branching characteristics. However, the establishment of structure-function relationships is possible only if purified and characterized polysaccharides are used and selective structural modifications performed. Aiming at contributing to the definition of the structure-function relationships necessary to design immunostimulatory polysaccharides with potential for preventive or therapeutical purposes or to be recognized as health-improving ingredients in functional foods, this review introduces basic immunological concepts required to understand the mechanisms that rule the potential claimed immunostimulatory activity of polysaccharides and critically presents a literature survey on the structural features of the polysaccharides and reported immunostimulatory activity. Copyright © 2015 Elsevier Ltd. All rights reserved.
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    • "The hemolytic complement-fixing test carried out in this study is a commonly used method for screening samples for their influence on the classical complement pathway [3]. The inhibition of hemolysis is a measure of the amount of complement that has reacted with the samples [19]. A pectic polysaccharide from B. umbraculum (BPII), used as a positive control, has previously been shown to be highly active in the complement-fixing assay [20,21]. "
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    • "Enhydra fluctuans was reported for antioxidant, analgesic, antimicrobial activity, and antibacterial activity23242526 . Glinus oppositifolius (L.) was found to contain immunomodulating peptic polymer, terpinoid saponine having antiprotozoal activity, and bioactives having antioxidant property272829 . Ipomoea aquatica Forsk was reported for hypoglycemic activity, cytotoxic activity303132 . "
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