Folate metabolism in plants - An Arabidopsis homolog of the mammalian mitochondrial folate transporter mediates folate import into chloroplasts

Department of Pharmacology and Toxicology, Virginia Commonwealth University, Ричмонд, Virginia, United States
Journal of Biological Chemistry (Impact Factor: 4.57). 11/2005; 280(41):34823-31. DOI: 10.1074/jbc.M506045200
Source: PubMed


The distribution of folates in plant cells suggests a complex traffic of the vitamin between the organelles and the cytosol. The Arabidopsis thaliana protein AtFOLT1 encoded by the At5g66380 gene is the closest homolog of the mitochondrial folate transporters (MFTs) characterized in mammalian cells. AtFOLT1 belongs to the mitochondrial carrier family, but GFP-tagging experiments and Western blot analyses indicated that it is targeted to the envelope of chloroplasts. By using the glycine auxotroph Chinese hamster ovary glyB cell line, which lacks a functional MFT and is deficient in folates transport into mitochondria, we showed by complementation that AtFOLT1 functions as a folate transporter in a hamster background. Indeed, stable transfectants bearing the AtFOLT1 cDNA have enhanced levels of folates in mitochondria and can support growth in glycine-free medium. Also, the expression of AtFOLT1 in Escherichia coli allows bacterial cells to uptake exogenous folate. Disruption of the AtFOLT1 gene in Arabidopsis does not lead to phenotypic alterations in folate-sufficient or folate-deficient plants. Also, the atfolt1 null mutant contains wild-type levels of folates in chloroplasts and preserves the enzymatic capacity to catalyze folate-dependent reactions in this subcellular compartment. These findings suggest strongly that, despite many common features shared by chloroplasts and mitochondria from mammals regarding folate metabolism, the folate import mechanisms in these organelles are not equivalent: folate uptake by mammalian mitochondria is mediated by a unique transporter, whereas there are alternative routes for folate import into chloroplasts.

Download full-text


Available from: Fabrice Rébeillé, Nov 24, 2015
    • "The mitochondrial carriers (MCs) constitute a family of eukaryotic intracellular transport proteins that, apart from a few exceptions (Palmieri et al. 2001b; Fukao et al. 2001; Bedhomme et al. 2005; Leroch et al. 2008; Bouvier et al. 2006; Thuswaldner et al. 2007; Kirchberger et al. 2008; Arai et al. 2008; Eubel et al. 2008; Linka et al. 2008; Palmieri et al. 2009; Agrimi et al. 2012), are localized to the inner membranes of mitochondria (Palmieri 2004, Abstract Among the members of the mitochondrial carrier family, there are transporters that catalyze the translocation of ornithine and related substrates, such as arginine, homoarginine, lysine, histidine, and citrulline, across the inner mitochondrial membrane. The mitochondrial carriers ORC1, ORC2, and SLC25A29 from Homo sapiens, BAC1 and BAC2 from Arabidopsis thaliana, and Ort1p from Saccharomyces cerevisiae have been biochemically characterized by transport assays in liposomes. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Among the members of the mitochondrial carrier family, there are transporters that catalyze the translocation of ornithine and related substrates, such as arginine, homoarginine, lysine, histidine, and citrulline, across the inner mitochondrial membrane. The mitochondrial carriers ORC1, ORC2, and SLC25A29 from Homo sapiens, BAC1 and BAC2 from Arabidopsis thaliana, and Ort1p from Saccharomyces cerevisiae have been biochemically characterized by transport assays in liposomes. All of them transport ornithine and amino acids with side chains terminating at least with one amine. There are, however, marked differences in their substrate specificities including their affinity for ornithine (KM values in the mM to μM range). These differences are most likely reflected by minor differences in the substrate binding sites of these carriers. The physiological role of the above-mentioned mitochondrial carriers is to link several metabolic pathways that take place partly in the cytosol and partly in the mitochondrial matrix and to provide basic amino acids for mitochondrial translation. In the liver, human ORC1 catalyzes the citrulline/ornithine exchange across the mitochondrial inner membrane, which is required for the urea cycle. Human ORC1, ORC2, and SLC25A29 are likely to be involved in the biosynthesis and transport of arginine, which can be used as a precursor for the synthesis of NO, agmatine, polyamines, creatine, glutamine, glutamate, and proline, as well as in the degradation of basic amino acids. BAC1 and BAC2 are implicated in some processes similar to those of their human counterparts and in nitrogen and amino acid metabolism linked to stress conditions and the development of plants. Ort1p is involved in the biosynthesis of arginine and polyamines in yeast.
    No preview · Article · May 2015 · Amino Acids
  • Source
    • "The matrix gate mutations include residues of the signature motif PX[DE]XX[RK] that are highly conserved in all carriers and can therefore not have a discriminatory role in substrate recognition (Figure 5B). Carriers transporting NAD + (Todisco et al. 2006, Palmieri et al. 2009), pyrimidine nucleotides (Marobbio et al. 2006, Floyd et al. 2007), FAD/ folate (Tzagoloff et al. 1996, Titus and Moran, 2000, Bedhomme et al. 2005), and coenzyme A, FAD and NAD + in peroxisomes (Agrimi et al. 2012a, 2012b, Bernhardt et al. 2012) have a conserved W instead of [DE] in the second signature motif; FAD/folate carriers and ATP-Mg 2+ /phosphate carriers (Fiermonte et al. 2004, Traba et al. 2008, 2009) have a glutamine and [QNAT], respectively, instead of the negatively charged residue of the third signature motif; dicarboxylate carriers (Palmieri et al. 1996b, 2008, Fiermonte et al. 1998) have an asparagine or methionine instead of the positively charged residue of the second motif; the fungal oxaloacetate/sulphate/ a-isopropylmalate carrier (Palmieri et al. 1999, Marobbio et al. 2008) lacks the negatively charged residue and the positively charged residue in the second and third motifs, the former residue being replaced by [FY] and the latter by [LM]; and the phosphate carriers (Runswick et al. 1987, Dolce et al. 1994, Wohlrab and Briggs 1994) have a hydrophobic substitution instead of the positively charged residue in the third motif. These mostly polar modifications would be capable to either cation-p or hydrogen bond interactions, which have an interaction energy that is approximately half of an ionic bond. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Abstract Mitochondrial carriers transport inorganic ions, nucleotides, amino acids, keto acids and cofactors across the mitochondrial inner membrane. Structurally they consist of three domains, each containing two transmembrane α-helices linked by a short α-helix and loop. The substrate binds to three major contact points in the central cavity. The class of substrate (e.g., adenine nucleotides) is determined by contact point II on transmembrane α-helix H4 and the type of substrate within the class (e.g., ADP, coenzyme A) by contact point I in H2, whereas contact point III on H6 is most usually a positively charged residue, irrespective of the type or class. Two salt bridge networks, consisting of conserved and symmetric residues, are located on the matrix and cytoplasmic side of the cavity. These residues are part of the gates that are involved in opening and closing of the carrier during the transport cycle, exposing the central substrate binding site to either side of the membrane in an alternating way. Here we revisit the plethora of mutagenesis data that have been collected over the last two decades to see if the residues in the proposed binding site and salt bridge networks are indeed important for function. The analysis shows that the major contact points of the substrate binding site are indeed crucial for function and in defining the specificity. The matrix salt bridge network is more critical for function than the cytoplasmic salt bridge network in agreement with its central position, but neither is likely to be involved in substrate recognition directly.
    Full-text · Article · Mar 2013 · Molecular Membrane Biology
  • Source
    • "online). MCF members are not confined to mitochondria but are also found in other cell compartments, namely, chloroplasts, peroxisomes , the endoplasmic reticulum, or the plasma membrane (Bedhomme et al., 2005; Bouvier et al., 2006; Thuswaldner et al., 2007; Kirchberger et al., 2008; Leroch et al., 2008; Linka et al., 2008; Palmieri et al., 2008, 2009; Rieder and Neuhaus, 2011; Bernhardt et al., 2012). They mediate the transport of various substrates, including nucleotides, mainly in an antiport manner (Palmieri et al., 2011). "
    [Show abstract] [Hide abstract]
    ABSTRACT: 3'-Phosphoadenosine 5'-phosphosulfate (PAPS) is the high-energy sulfate donor for sulfation reactions. Plants produce some PAPS in the cytosol, but it is predominantly produced in plastids. Accordingly, PAPS has to be provided by plastids to serve as a substrate for sulfotransferase reactions in the cytosol and the Golgi apparatus. We present several lines of evidence that the recently described Arabidopsis thaliana thylakoid ADP/ATP carrier TAAC transports PAPS across the plastid envelope and thus fulfills an additional function of high physiological relevance. Transport studies using the recombinant protein revealed that it favors PAPS, 3'-phosphoadenosine 5'-phosphate, and ATP as substrates; thus, we named it PAPST1. The protein could be detected both in the plastid envelope membrane and in thylakoids, and it is present in plastids of autotrophic and heterotrophic tissues. TAAC/PAPST1 belongs to the mitochondrial carrier family in contrast with the known animal PAPS transporters, which are members of the nucleotide-sugar transporter family. The expression of the PAPST1 gene is regulated by the same MYB transcription factors also regulating the biosynthesis of sulfated secondary metabolites, glucosinolates. Molecular and physiological analyses of papst1 mutant plants indicate that PAPST1 is involved in several aspects of sulfur metabolism, including the biosynthesis of thiols, glucosinolates, and phytosulfokines.
    Full-text · Article · Oct 2012 · The Plant Cell
Show more