Presence of CRF09_cpx and complex CRF02_AG/CRF09_cpx recombinant HIV type 1 strains in Cote d'Ivoire, West Africa

PAC-CI, Abidjan, Côte d'Ivoire.
AIDS Research and Human Retroviruses (Impact Factor: 2.33). 08/2005; 21(7):667-72. DOI: 10.1089/aid.2005.21.667
Source: PubMed


Based on partial env and pol (protease and RT) subtyping, we recently documented that the majority (>80%) of the HIV-1 strains that circulate in Côte d'Ivoire were CRF02_AG and about 11% were recombinants or could not be clearly assigned to a known subtype or CRF. In order to determine in more detail the precise structure of these viruses we sequenced the full-length genomes for six such strains. Bootscan and phylogenetic tree analysis showed that four strains were complex and unique CRF02_AG/CRF09_cpx recombinants, one was a CRF02_AG/CRF06_cpx recombinant, and one was a pure CRF09_cpx. Reanalysis of the remaining recombinants asserted the predominance of CRF09_cpx within intersubtype recombinants and circulation of CRF09_cpx in Côte d'Ivoire. More detailed analysis of the CRF09_cpx strains revealed also that part of the pol gene belonged to subtype K. This is the first time that such recombinants are described.

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    • "Group M can be further subdivided into subtypes (A-D, F-H, J-K) and sub-subtypes (A1-A4, F1-F2). In addition an increasing number of Circulating Recombinant Forms (CRFs, CRF01-CRF37) and many Unique Recombinants Forms (URFs) have also been described [4-6]. The geographic distribution of the different HIV-1 M variants is very heterogeneous and specific distributions of the various subtypes are seen among the different continents, even from country to country or within countries [7]. "
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