Morphological, ontogenetic and molecular characterization of Scutellospora reticulata (Glomeromycota). Mycol Res

Université catholique de Louvain, Лувен-ла-Нев, Wallonia, Belgium
Mycological Research (Impact Factor: 2.81). 07/2005; 109(Pt 6):697-706. DOI: 10.1017/S0953756205002546
Source: PubMed


The arbuscular mycorrhizal (AM) fungus Scutellospora reticulata (CNPAB11) was characterized using morphological, ontogenetic and molecular approaches. Spore ontogenesis was studied using Ri T-DNA transformed carrot roots and observations were compared with those published for eight other, pot-cultured, Scutellospora species. The sporogenesis of S. reticulata exhibited an unreported pattern of outer spore wall differentiation. In addition, Denaturing Gradient Gel Electrophoresis (DGGE), targeting the V9 region of the SSU nrDNA, was used to differentiate S. reticulata from 16 other Scutellospora species and results were confirmed by sequencing analysis. Phylogenetic analyses, using nearly full length SSU nrDNA sequences, grouped S. reticulata in a cluster together with S. cerradensis and S. heterogama, species that share similar spore wall organization and also possess ornamented external walls. PCR-DGGE and sequence analysis revealed intragenomic SSU nrDNA polymorphisms in four out of six Scutellospora species tested, and demonstrated that SSU nrDNA intragenomic polymorphism could be used as a marker to differentiate several closely related Scutellospora species.

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Available from: Francisco Adriano de Souza
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    • "An advantage of using root organ culture as a system is that both symbionts (AM and host root) are grown and easily maintained on a defined medium, allowing nondestructive in vivo observations over long periods of time (de Souza and Berbara 1999). The system has afforded studies on organization and development of mycelium (Bago et al. 1998a), sporulation dynamics (Declerck et al. 2001, 2004; Voets et al. 2009; Ijdo et al. 2011), and spore ontogeny (de Souza et al. 2005). Further potential uses of the system are the production of pure and concentrated inoculum, and contaminant-free fungal tissues for genetic and physiological studies. "
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    ABSTRACT: Spores of arbuscular mycorrhizal (AM) fungus Funneliformis mosseae (Nicolson & Gerdemann) Walker & Schuessler were cultured in association with transformed roots of Linum usitatissimum L. (Linaceae) for the first time on modified Strullu-Romand medium (pH 5.5) in monoxenic culture. Germ tubes emerged through the spore wall in 88% of spores after 5 days. Hyphal contact with transformed linum roots was observed 5 days after co-cultivation. Paris-type arbuscules and hyphal coils were seen. Extra-radical branched absorbing structures were common. Terminal and intercalary secondary spores were also formed. Spore viability when assessed using vital dye staining (MTT test) was 83%. Secondary spores that proved viable were subsequently transferred from in vitro to in vivo culture where Arum-type arbuscules, intra-radical intercellular hyphae, and extra-radical spores were observed. The procedure established shows potential in AM inoculum mass production and possibility in application.
    Full-text · Article · Oct 2015 · Mycology
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    • "Arbuscular mycorrhizal fungi have been classified within the classic life-history strategies 'r–K' on the basis of sporulation traits (De Souza et al. 2005; Ijdo et al. 2010; Verbruggen and Kiers 2010). While the AMF of the Gigasporaceae (with the gigasporoid morphotype) produce few and large spores within a long life cycle, and tend "
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    ABSTRACT: Arbuscular-mycorrhizal internal structures (i.e. total root colonization, arbuscules, vesicles) and external structures (i.e. spore density), and Glomeromycota spore morphotypes, were evaluated in wheat severely infected with Mycosphaerella graminicola - the causal agent of Septoria leaf blotch. Plots in which the infection was controlled with a commercial fungicide at recommended field doses, were also examined. The commercial fungicide used was an admixture of trifloxistrobin and tebuconazole. No negative effects of the fungicide application on arbuscular-mycorrhizal fungi (AMF) were found. The M. graminicola fungicidal treatment actually favoured the formation of arbuscules and AMF spores, as there was a selective increase in the density of spores belonging to the glomoid morphotype. Arbuscular-mycorrhizal fungi have an absolute dependence on the carbon provided by the plant. A severe foliar disease leading to a diminished carbon supply to the roots would generate decreases in carbon availability. Such decreases would strongly affect mycorrhizal associations and development. Furthermore, the change in the green-leaf area produced by a severe foliar disease and/or a reversal of that condition through fungicide treatment could result in shifts in the composition of the AMF community so as to favour glomoid morphotypes. Glomoid species have been previously considered as r-strategists
    Full-text · Article · Jan 2014
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    • "The data originate from the following publications: Simon et al. (1992, 1993a,b), Gehrig et al. (1996), Simon (1996), Helgason et al. (1998), Sawaki et al. (1998), Vandenkoornhuyse & Leyval (1998), Helgason et al. (1999), Declerck et al. (2000), Kramadibrata et al. (2000), Redecker et al. (2000a,b), Daniell et al. (2001), Schü ßler et al. (2001a,b), Schwarzott et al. (2001), Bidartondo et al. (2002), Helgason et al. (2002), Husband et al. (2002a,b), Kowalchuk et al. (2002), Vandenkoornhuyse et al. (2002a,b), Helgason et al. (2003), O ¨ pik et al. (2003), Regvar et al. (2003), Calvente et al. (2004), Ferrol et al. (2004), Haug et al. (2004), Heinemeyer et al. (2004), Oba et al. (2004), Saito et al. (2004), Scheublin et al. (2004), Walker et al. (2004), Whitfield et al. (2004), Wirsel (2004), de Souza et al. (2005), Douhan et al. (2005), Jumpponen et al. (2005), Ma et al. (2005), O'Brien et al. (2005), Rowe & Pringle (2005), Russell & Bulman (2005), Sato et al. (2005), Yamato & Iwase (2005), de la Peñ a et al. (2006), DeBellis & Widden (2006), Franke et al. (2006), James et al. (2006), Martynova-Van Kley et al. (2006), Rodríguez-Echeverría & Freitas (2006), Santos et al. (2006), Vallino et al. (2006), Wubet et al. (2006a,b), Beck et al. (2007), Helgason et al. (2007), Ková cs et al. (2007), Ligrone et al. (2007), Porras-Alfaro et al. (2007), Redecker et al. (2007), Renker et al. (2007), Santos-Gonzá lez et al. (2007), Walker et al. (2007), Vandenkoornhuyse et al. (2007), Winther & Friedman (2007), Alguacil et al. (2008), Appelhans et al. (2008), Błaszkowski et al. (2008), Burke (2008), Kottke et al. (2008), Lee et al. (2008), Lesaulnier et al. (2008), Liang et al. (2008), Likar et al. (2008), Maki et al. (2008) "
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    ABSTRACT: • Here, we describe a new database, MaarjAM, that summarizes publicly available Glomeromycota DNA sequence data and associated metadata. The goal of the database is to facilitate the description of distribution and richness patterns in this group of fungi. • Small subunit (SSU) rRNA gene sequences and available metadata were collated from all suitable taxonomic and ecological publications. These data have been made accessible in an open-access database ( • Two hundred and eighty-two SSU rRNA gene virtual taxa (VT) were described based on a comprehensive phylogenetic analysis of all collated Glomeromycota sequences. Two-thirds of VT showed limited distribution ranges, occurring in single current or historic continents or climatic zones. Those VT that associated with a taxonomically wide range of host plants also tended to have a wide geographical distribution, and vice versa. No relationships were detected between VT richness and latitude, elevation or vascular plant richness. • The collated Glomeromycota molecular diversity data suggest limited distribution ranges in most Glomeromycota taxa and a positive relationship between the width of a taxon's geographical range and its host taxonomic range. Inconsistencies between molecular and traditional taxonomy of Glomeromycota, and shortage of data from major continents and ecosystems, are highlighted.
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