Antinociceptive activity of alcoholic extract of Hemidesmus indicus R.Br. in mice

J.L. Chaturvedi College of Pharmacy, Ajni, Maharashtra, India
Journal of Ethnopharmacology (Impact Factor: 3). 12/2005; 102(2):298-301. DOI: 10.1016/j.jep.2005.05.039
Source: PubMed


The ethanolic extract of roots of Hemidesmus indicus R.Br. (family: Asclepiadaceae) was investigated for possible antinociceptive effect in mice. Three models were used to study the effects of extracts on nociception, which was induced, by acetic acid (Writhing test), formalin (Paw licking test) and hot plate test in mice. Hemidesmus indicus R.Br. extract was administered in the dose range of 25, 50 and 100mg/kg orally 1h prior to pain induction. The preliminary phytochemical screening of the extract showed the presence of triterpenes, flavonoids, pregnane glycosides and steroids. Oral administration of Hemidesmus indicus extract revealed dose-dependent antinociceptive effect in all the models for antinociception and it blocked both the neurogenic and inflammatory pain and the nociceptive activity was comparable with the reference drug. The results indicate that alcoholic extract of Hemidesmus indicus R.Br. possesses a significant antinociceptive activity. The activity can be related with the significant phytochemicals such as triterpenes, flavonoids, and sterols reported in the root extract.

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    • "Traditionally it is being used for the treatment of epileptic fits in children.[6] Methanolic roots extract exhibited significant (P < 0.05) nociception at a dose of 50 mg/kg in mice.[11] Even the methanolic root extract of H. indicus was also proven to be an effective neuro-protectant and reduce the cerebral infraction when tested in rats. "
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    ABSTRACT: Background:The plants selected for the study were traditionally used in siddha system of medicine in neurological disorders.Aim:The aim of the following study isto screen the plant species for both acetylcholinesterase (AchE) and butyrylcholinesterase (BuchE) inhibition by in-vitro Ellman's method and a thin layer chromatography bioautographic assay for newer drug candidates for the treatment of Alzheimer's disease.Materials and Methods:Ellman's colorimetric method was performed in a 96 well micro plate for cholinesterases inhibition using galantamine as standard drug.Results:Present studies confirmed that out of all the tested extracts Hemidesmus indicus R.Br (HI) showed considerable IC50 values for AchE (28.40 ± 0.92 μg/mL) and BuchE (43.47 ± 0.64 μg/mL) inhibition which indicates that HI extract has considerable specificity toward AchE and BuchE compared with all the tested extracts and the activity was followed by Vernonia anthelmintica (VA) Willd and Saussurea lappa Clarke (SL). The bioautograms also confirmed the activity potent extracts.Conclusion:Besides various bioactivities HI, VA and SL exhibited considerable cholinesterases inhibition making it to consider these species for further investigation of new compounds.
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    • "direct action of formalin on nociceptors within the intraplantar region. Meanwhile, the late phase, which appears between 15 and 60 min after the phlogistic agent administration, is considered as an inflammatory-mediated pain resulting from a tonic response due to the release of inflammatory mediators [20] and activation of the neurons in the dorsal horns of the spinal cord [21] [22]. Other advantages of this assay include its ability to verify the potential of new compounds/extracts to affect noninflammatory or inflammatory-associated pain. "
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    • "This model evaluates two distinct phase of nociception. The first phase, classified as a neurogenic pain, is an acute response observed immediately after the administration of formalin , persisting for 5 min and that occurs through direct chemical stimulation promoted by formalin on nociceptors, in type C and part of the Aδ afferent fibers; it is also associated with the release of excitatory amino acids, as glutamate, aspartate, taurine and glycine that are known to be involved in the transmission of peripheral nociception (Verma et al., 2005; Malmberg and Yaksh, 1995). The second phase appears between 15 and 60 min after the formalin administration, classified as an inflammatory pain, and is a tonic response associated with the release of chemical mediators such as histamine, serotonin, bradykinin, prostaglandins and excitatory amino acids (Sani et al., 2012). "
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