Article

Genetic analysis of the populations from Northern and Mesopotamian provinces of Argentina by means of 15 autosomal STRs

Servicio de Huellas Digitales Genéticas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113-Buenos Aires, Argentina.
Forensic Science International (Impact Factor: 2.14). 08/2006; 160(2-3):224-30. DOI: 10.1016/j.forsciint.2005.07.006
Source: PubMed

ABSTRACT

The allele frequencies for the 15 short tandem repeats included in the Power Plex-16 kit (Promega Corp., Madison, WI, USA) were determined in a sample of 429 unrelated individuals from five provinces of the Northern and Northeastern regions of Argentina. Three Northern provinces including Salta, Formosa and Chaco and two within the region surrounded by the Paraná and Uruguay Rivers commonly known as the Argentine Mesopotamia, including Misiones and Corrientes. Since in this region Entre Ríos Province is also present, previously published results were used for comparison. The calculated parameters: polymorphism information content (PIC); discrimination power (DP); matching probability (MP); typical paternity index (TPI) and power of exclusion (PE) showed Penta E to be the most valuable marker from the studied sample set. All loci met Hardy-Weinberg expectations using the Bonferroni correction for the number of loci analyzed, except D3S1358 in Salta and THO1 in Formosa provinces. Population differentiation test revealed that the Salta population sample data denoted significant differences for various loci when compared with the other province information presented here in, as well as with other published data sets.

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Available from: Daniel Corach, Dec 29, 2015
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    • "Para las 16 poblaciones, los valores cofenéticos indican " pobre ajuste " (r = 0,7795) con Nei '72, " buen ajuste " (r = 0,8336) con Nei '78 y " muy buen ajuste " (r = 0,9430) con Prevosti (Fig. 4). Llama la atención el alejamiento de la muestra Salta (Marino et al., 2006a) con respecto a todas las localidades de la provincia, como también la de Pampa Grande (excepto en Nei '72). Con respecto al coefi ciente BAND (Fig. 5), se dispersan todas las localidades de la provincia en el conjunto país sin una clara proximidad genética entre ellas. "

    Full-text · Article · Mar 2011
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    • "In 2004, our group developed and released the first on-line reference database on autosomal STRs allele and Y-STRs haplotype frequencies for the Argentinean population [1] [2] [3] [4]. "
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    ABSTRACT: Allele frequency data of genetic markers employed in paternity testing and forensic casework is essential for the statistical analysis of the results obtained by these molecular markers.In 2004, our group developed and launched a free on-line reference database including autosomal STRs allele and Y-STR haplotype frequencies for 10 Argentinean provinces (http://www.ffyb.uba.ar/marcadores/antecedentes.asp). The dataset included 2710 individuals typed by means of 13 autosomal STRs and 239 individuals with the minimal Y-STRs haplotype (YHRD).In order to further improve the available information on-line, the database was increased for both autosomal and Y-STR markers. A total of 6501 genotypes from unrelated donors were added to the previous database. These samples were obtained from Buenos Aires, Chubut, Rio Negro, Santa Fe, Mendoza, Misiones, Corrientes, Chaco, Formosa and Salta, and typed at least with 13 autosomal STRs and 1336 unrelated males were also typed for Y-STRs. In parallel, protocols for DNA analysis and reference bibliography were updated.The present update attained a total of 9211 genotypes and 1575 Y-chromosome haplotypes from the Argentinean population. This contribution offers on-line statistical information about highly standardized genetic markers to the forensic and molecular anthropology research communities.
    Full-text · Article · Dec 2009 · Forensic Science International Genetics Supplement Series
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    ABSTRACT: Tese (doutorado)—Universidade de Brasília, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Biologia Animal, 2008. A América Latina tem sido palco de grandes transformações populacionais, sendo hoje caracterizada por ter uma constituição multiétnica, porém com maior influência de três populações parentais - européia, africana e ameríndia. Apesar das similaridades, cada país latino-americano apresentou uma história de povoamento singular, já que ocorreram variações na distribuição das populações parentais e na quantidade de fluxo gênico entre elas. Esse trabalho teve por objetivo avaliar a constituição genética de populações miscigenadas da América Latina e Caribe e a estruturação genética entre elas. Para tanto, foram considerados quatro conjuntos populacionais e dois conjuntos de marcadores. Primeiro, foram analisados treze países da América Latina e Caribe (Argentina, Bahamas, Brasil, Chile, Colômbia, Costa Rica, El Salvador, Equador, Jamaica, México, Peru, Porto Rico e Venezuela), utilizando marcadores do tipo STRs (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX e vWA). Em um segundo momento, foram avaliadas as diferenças entre as regiões geográficas de três países da América do Sul (Argentina, Brasil e Colômbia) utilizando os mesmos marcadores. Para uma análise mais regional, populações urbanas da região centro-oeste brasileira foram analisadas utilizando os mesmo marcadores anteriores adicionados do Penta E. O povoamento da região centrooeste foi também avaliado pela comparação de duas populações da região – Goiás e Distrito Federal – utilizando marcadores AIMs autossômicos - APO, AT3, D1, DRD2- A, ECA, FXIIIB, FyNull, GC, LPL, OCA2, PV92, Rb2300, Sb19.3 e TPA25. As análises de distância genética entre as populações latino-americanas e entre as regiões dos três países mostraram um quadro bastante heterogêneo quanto à distribuição das freqüências alélicas dos marcadores utilizados. A análise de mistura corroborou os dados de distância, sendo que a contribuição parental ameríndia variou de 5% a 73%, a africana de 4% a 90% e a européia de 4% a 66%. Com relação à análise das populações da região centro-oeste do Brasil, não foi observado diferenciação entre a distribuição gênica e genotípica, provavelmente devido ao intenso fluxo gênico entre elas. A mistura gênica estimada, considerando os marcadores microssatélites, foi de 11% para a parental ameríndia, 21% para a parental africana e de 68% para a parental européia. Os resultados obtidos com os marcadores AIMs foi de 15%, 21% e 63%, respectivamente. A maioria das populações analisadas apresentou modelo tri-híbrido de mistura. Esses resultados são concordantes com dados históricos e demográficos das populações latino-americanas. ____________________________________________________________________________ ABSTRACT The Latin American population has been under great transformations and today it can be characterized as having a multiethnic constitution, however with three main parental populations influence - European, African and Amerindian. Despite their similarities, each country has a unique history of settlement, since there have been variations in the distribution of the parental populations and in the amount of gene flow between them. This study aimed to evaluate the genetic constitution in Latin American and Caribbean populations and the genetic structure between them. For that matter, four groups of populations and two sets of markers were employed. First, thirteen countries of Latin America and Caribbean (Argentina, Bahamas, Brazil, Chile, Colombia, Costa Rica, El Salvador, Ecuador, Jamaica, Mexico, Peru, Puerto Rico and Venezuela), were analyzed with STR markers (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX and vWA). In a second moment, the differences between the geographic regions of three South-American countries were evaluated (Argentina, Brazil and Colombia) using the same markers. Next, for a more regional analysis, urban populations from Brazilian Middle-Western region were studied using the same markers listed above and Penta E. The settlement of the Brazilian Middle-Western region was also evaluated regarding the comparison of two populations of this region - Goiás and Federal District - using autosomic AIMs - APO, AT3, D1, DRD2-A, ECA, FXIIIB, FyNull, GC, LPL, OCA2, PV92, Rb2300, SB19.3 and TPA25. The analyses of the genetic distance between the Latin American populations and between the regions of the three countries had shown a heterogeneous picture of the distribution of the allelic frequencies. The admixture analysis corroborated the genetic distance data, with the Amerindian parental contribution presenting variations ranging from 5 to 73%, the African from 4 to 90% and the European from 4 to 66%. Regarding the analysis of the populations of the Brazilian Middle-Western region, differentiation between the genetic and genotypic distribution was not observed, probably due the intense gene flow between them. The genetic admixture estimate, considering the STRs markers, was 11% for the Amerindian parental, 21% for the African parental and 68% for the European parental. The results generated with the AIMs were 15%, 21% and 63%, respectively. The majority of the analyzed populations are tri-hybrid, having been formed by three parental groups. These results agree with historical and demographic data of the Latin American populations.
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