The Zinc-Finger Protein Zat12 Plays a Central Role in Reactive Oxygen and Abiotic Stress Signaling in Arabidopsis

George Mason University, 페어팩스, Virginia, United States
Plant physiology (Impact Factor: 6.84). 11/2005; 139(2):847-56. DOI: 10.1104/pp.105.068254
Source: PubMed


Plant acclimation to environmental stress is controlled by a complex network of regulatory genes that compose distinct stress-response regulons. In contrast to many signaling and regulatory genes that are stress specific, the zinc-finger protein Zat12 responds to a large number of biotic and abiotic stresses. Zat12 is thought to be involved in cold and oxidative stress signaling in Arabidopsis (Arabidopsis thaliana); however, its mode of action and regulation are largely unknown. Using a fusion between the Zat12 promoter and the reporter gene luciferase, we demonstrate that Zat12 expression is activated at the transcriptional level during different abiotic stresses and in response to a wound-induced systemic signal. Using Zat12 gain- and loss-of-function lines, we assign a function for Zat12 during oxidative, osmotic, salinity, high light, and heat stresses. Transcriptional profiling of Zat12-overexpressing plants and wild-type plants subjected to H(2)O(2) stress revealed that constitutive expression of Zat12 in Arabidopsis results in the enhanced expression of oxidative- and light stress-response transcripts. Under specific growth conditions, Zat12 may therefore regulate a collection of transcripts involved in the response of Arabidopsis to high light and oxidative stress. Our results suggest that Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis.

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Available from: Sholpan Davletova
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    • "Finally, WRKY, as one of plant-specific TFs, contained one large family of regulatory protein in plants444546, which participated in various biotic stress response and several developmental and physiological processes474849505152. Some WRKYs in A. thaliana were significantly enhanced by H 2 O 2 , which was one specific ROS5354555657585960. These indicated WRKY maybe perform an important role under oxidative stress. "
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    • "S-deficiency and some environmental conditions such as salt stress and AgNO 3 -treatment were reported to induce the LSU1 and LSU2 genes (Zimmermann et al., 2004Zimmermann et al., , 2008). LSU2 gene was also induced by oxidative stress (Davletova et al., 2005), carbon starvation and in response to sugar (Usadel et al., 2008). In addition, Arabidopsis LSU2 gene was temporarily determined as one of the genes cross-talking between several signals pathways such as iron, sulfur, nitrate and hormones under various stress conditions including sulfur and iron restriction, and nitrate and stress hormone treatments (Omranian et al., 2012). "
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