Article

Characterization of a Novel Glucosamine-6-Phosphate Deaminase from a Hyperthermophilic Archaeon

Kwansei Gakuin University, Nishinomiya, Hyōgo, Japan
Journal of Bacteriology (Impact Factor: 2.81). 11/2005; 187(20):7038-44. DOI: 10.1128/JB.187.20.7038-7044.2005
Source: PubMed

ABSTRACT

A key step in amino sugar metabolism is the interconversion between fructose-6-phosphate (Fru6P) and glucosamine-6-phosphate (GlcN6P). This conversion is catalyzed in the catabolic and anabolic directions by GlcN6P deaminase and GlcN6P synthase, respectively, two enzymes that show no relationship with one another in terms of primary structure. In this study, we examined the catalytic properties and regulatory features of the glmD gene product (GlmD(Tk)) present within a chitin degradation gene cluster in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. Although the protein GlmD(Tk) was predicted as a probable sugar isomerase related to the C-terminal sugar isomerase domain of GlcN6P synthase, the recombinant GlmD(Tk) clearly exhibited GlcN6P deaminase activity, generating Fru6P and ammonia from GlcN6P. This enzyme also catalyzed the reverse reaction, the ammonia-dependent amination/isomerization of Fru6P to GlcN6P, whereas no GlcN6P synthase activity dependent on glutamine was observed. Kinetic analyses clarified the preference of this enzyme for the deaminase reaction rather than the reverse one, consistent with the catabolic function of GlmD(Tk). In T. kodakaraensis cells, glmD(Tk) was polycistronically transcribed together with upstream genes encoding an ABC transporter and a downstream exo-beta-glucosaminidase gene (glmA(Tk)) within the gene cluster, and their expression was induced by the chitin degradation intermediate, diacetylchitobiose. The results presented here indicate that GlmD(Tk) is actually a GlcN6P deaminase functioning in the entry of chitin-derived monosaccharides to glycolysis in this hyperthermophile. This enzyme is the first example of an archaeal GlcN6P deaminase and is a structurally novel type distinct from any previously known GlcN6P deaminase.

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    • "Proteins with SIS domains have been classified in the Cluster of Orthologous Group of proteins as COG222. It was proposed by Tanaka and co-workers that although AgaI has sequence homology to nagB encoded GlcNAc-6-P deaminase/isomerase and has been predicted to be the Gam-6-P deaminase/isomerase, AgaS which belongs to COG222 could be an additional Gam-6-P deaminase [23]. Based on these reports and our findings that neither agaI nor nagB has a role in Aga and Gam utilization, we propose that agaS codes for Gam-6-P deaminase/isomerase. "
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