Simultaneous determination of cannabidiol, cannabinol, and Δ9-tetrahydrocannabinol in human hair by gas chromatography-mass spectrometry

Drug Analysis Laboratory, Forensic Science Division, Supreme Prosecutors' Office, Seocho-dong, Seocho-gu, Seoul 137-730, Korea.
Archives of Pharmacal Research (Impact Factor: 2.05). 09/2005; 28(9):1086-91. DOI: 10.1007/BF02977406
Source: PubMed


An analytical method was developed for evaluating the cannabidiol (CBD), cannabinol (CBN), and delta9-tetrahydrocannabinol (delta9-THC) level in human hair using gas chromatography-mass spectrometry (GC-MS). Hair samples (50 mg) were washed with isopropyl alcohol and cut into small fragments (< 1 mm). After adding a deuterated internal standard, the hair samples were incubated in 1.0 M NaOH for 10 min at 95 degrees C. The analytes from the resulting hydrolyzed samples were extracted using a mixture of n-hexane-ethyl acetate (75:25, v/v). The extracts were then evaporated, derivatized, and injected into the GC-MS. The recovery ranges of CBD, CBN, and delta9-THC at three concentration levels were 37.9-94.5% with good correlation coefficients (r2 >0.9989). The intra-day precision and accuracy ranged from -9.4% to 17.7%, and the inter-day precision and accuracy ranged from -15.5% to 14.5%, respectively. The limits of detection (LOD) for CBD, CBN, and delta9-THC were 0.005, 0.002, and 0.006 ng/mg, respectively. The applicability of this method of analyzing the hair samples from cannabis abusers was demonstrated.

    • "It has been shown that CBD is not stable under the severe conditions of alkaline digestion [106] ; other issues include large volume of organic solvent used in traditional LLE procedures tedious sample preparation and poor reproducibility. Enzymatic digestion [114] and direct methanol extraction [94] [96] [115] have been also reported but require more time, up to 5 h. "
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