Expansion of mesenchymal stem cells isolated from pediatric and adult donor bone marrow. J Cell Biochem

Department of Pediatrics, Regina Margherita Children's Hospital, University of Turin, Turin, Italy.
Journal of Cellular Biochemistry (Impact Factor: 3.26). 03/2006; 97(4):744-54. DOI: 10.1002/jcb.20681
Source: PubMed


The enormous plasticity of mesenchymal stem cells (MSCs) suggests an improvement of a standard protocol of isolation and ex vivo expansion for experimental and clinical use. We isolated and expanded MSCs from bone marrow (BM) of pediatric and young adult donors, to analyze the growth kinetic, immunophenotype, telomere length, karyotype during ex vivo expansion. Seventeen BM samples were collected from young adult donors and 8 from pediatric donors. MSCs isolated from two groups showed no morphological differences while their cell growth was strictly related to the donor's age. The MSCs isolated from pediatric donors reached a cumulative PD almost twice as high as MSCs isolated from young adult donors after 112 days (10.2 +/- 1.9 versus 5.5 +/- 3.7). Furthermore, we analyzed the modulation of antigen expression in the MSCs isolated from two groups until 10th passage (77 days) and there was no significant difference between the modulation of antigen expression. In particular, at the first passage, MSCs showed a low contamination of hemopoietic cells which became insignificant in the following passages. There was a high expression of CD90, CD29, CD44 and CD105 and variable and moderate expression of CD166 and CD106 at the start of MSC culture and at each passage during expansion. No chromosomal alteration or evidence of cellular senescence were observed in all analyzed samples. All these data suggest that MSCs can be isolated and expanded from most healthy donors, providing for an autologous source of stem cells.

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    • "Furthermore , these cells had 78 chromosomes (76 autosomal and 2 sexual) (Langford et al. 1996; Mareschi et al. 2006). In this study, by assessing the first and eighth passages, no chromosomal disorders was observed in agreement with Mareschi et al. (2006) that evaluated the passages 2, 5, and 10 of human MSCs. In addition, karyotypes of the monkey BM-MSCs (Berman et al. 2010), placenta-derived MSCs in a woman (Semenov et al. 2010), endometrial MSCs in heifer (Mehrabani et al. 2014b), and Guinea pig fetal fibroblast cells (Mehrabani et al. 2014a) were normal and that of chromosomal analysis revealed no chromosomal abnormalities. "
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    • "In our study, passage 3 BM-MSCs obtained from different aged donors showed similar morphology in terms of their surface antigen characteristics. These results were in accordance with the study of Mareschi et al. (2006) where BM-MSCs of early adult donors (range of age 20–50) and pediatric donors (range of age 6–11) were compared until passage 10. The study by Huang et al. (2005) demonstrated that BM-MSCs of fetuses, 0–20-, 20–40-, and >40-year-old donors, had similar morphology and antigenic phenotype. "
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