Article

Rapid Multiplex Single Nucleotide Polymorphism Genotyping Based on Single Base Extension Reactions and Color-Coded Beads

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-0012, Japan.
Journal of Bioscience and Bioengineering (Impact Factor: 1.88). 02/2002; 94(4):368-70. DOI: 10.1016/S1389-1723(02)80180-3
Source: PubMed

ABSTRACT

A single nucleotide polymorphism (SNP) typing method using color-coded beads is promising because it is easy to use and inexpensive. However, the present protocols are not suitable for clinical and diagnostic applications because they need centrifugation for bead-washing. Here, we developed a simplified protocol without a bead-washing procedure that enables SNP typing of PCR amplified fragments in only 30 min.

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