Article

High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L). Theor Appl Genet

Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines.
Theoretical and Applied Genetics (Impact Factor: 3.79). 02/2006; 112(2):288-97. DOI: 10.1007/s00122-005-0127-8
Source: PubMed

ABSTRACT

Brown planthopper (BPH) is a destructive insect pest of rice in Asia. Identification and the incorporation of new BPH resistance genes into modern rice cultivars are important breeding strategies to control the damage caused by new biotypes of BPH. In this study, a major resistance gene, Bph18(t), has been identified in an introgression line (IR65482-7-216-1-2) that has inherited the gene from the wild species Oryza australiensis. Genetic analysis revealed the dominant nature of the Bph18(t) gene and identified it as non-allelic to another gene, Bph10 that was earlier introgressed from O. australiensis. After linkage analysis using MapMaker followed by single-locus ANOVA on quantitatively expressed resistance levels of the progenies from an F2 mapping population identified with marker allele types, the Bph18(t) gene was initially located on the subterminal region of the long arm of chromosome 12 flanked by the SSR marker RM463 and the STS marker S15552. The corresponding physical region was identified in the Nipponbare genome pseudomolecule 3 through electronic chromosome landing (e-landing), in which 15 BAC clones covered 1.612 Mb. Eleven DNA markers tagging the BAC clones were used to construct a high-resolution genetic map of the target region. The Bph18(t) locus was further localized within a 0.843-Mb physical interval that includes three BAC clones between the markers R10289S and RM6869 by means of single-locus ANOVA of resistance levels of mapping population and marker-gene association analysis on 86 susceptible F2 progenies based on six time-point phenotyping. Using gene annotation information of TIGR, a putative resistance gene was identified in the BAC clone OSJNBa0028L05 and the sequence information was used to generate STS marker 7312.T4A. The marker allele of 1,078 bp completely co-segregated with the BPH resistance phenotype. STS marker 7312.T4A was validated using BC2F2 progenies derived from two temperate japonica backgrounds. Some 97 resistant BC2F2 individuals out of 433 screened completely co-segregated with the resistance-specific marker allele (1,078 bp) in either homozygous or heterozygous state. This further confirmed a major gene-controlled resistance to the BPH biotype of Korea. Identification of Bph18(t) enlarges the BPH resistance gene pool to help develop improved rice cultivars, and the PCR marker (7312.T4A) for the Bph18(t) gene should be readily applicable for marker-assisted selection (MAS).

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Available from: Ji-Ung Jeung, Jan 14, 2014
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    • "QTL mapping located qBph3 to the short arm of chromosome 6 between RM589 and RM588 based on KDML105 × Rathu Heenati (Jairin et al. 2009) and PTB33 × RD6 (Jairin et al. 2007) crossings. Other BPH resistance QTLs have also been reported, including Bph17 on chromosome 4 (Sun et al. 2005), Bph4 on chromosome 6 (Kawaguchi et al. 2001; Sun et al. 2006) and Bph18 on chromosome 12 (Jena et al. 2006). In addition to resistance QTLs, a putative sesquiterpene synthase (TPS) gene (Os04g27430) was identified using SFP mapping with isogenic lines derived from the backcross of RH and KDML105 (Kamolsukyunyong et al. 2013). "
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    ABSTRACT: Pyramiding multiple genes into a desirable genetic background can take years to accomplish. In this paper, a pseudo-backcrossing scheme was designed to shorten the backcrossing cycle needed. PinK3, an aromatic and potentially high-yielding rice variety-although one that is intolerant to flash flooding (Sub) and susceptible to bacterial leaf blight (BB), leaf-neck blast (BL) and the brown planthopper (BPH)-was used as a genetic basis for significant improvements through gene pyramiding. Four resistance donors with five target genes (Sub1A-C, xa5, Xa21, TPS and SSIIa) and three QTLs (qBph3, qBL1 and qBL11) were backcrossed individually using markers into the pseudo-recurrent parent 'PinK3' via one cycle of backcrossing followed by two cycles of pseudo-backcrossing and three selfings with rigorous foreground marker-assisted selection. In total, 29 pseudo-backcross inbred lines (BILs) were developed. Genome composition was surveyed using 61 simple sequence repeats (SSRs), 35 of which were located on six carrier chromosomes, with the remainder located on six non-carrier chromosomes. The recurrent genome content (%RGC) and donor genome content (%DGC), which were based on the physical positions of BC1F2, ranged from 69.99 to 88.98% and 11.02 to 30.01%, respectively. For the pseudo-BC3F3BILs, the %RGC and %DGC ranged from 74.50 to 81.30% and 18.70 to 25.50%, respectively. These results indicated that without direct background selection, no further increases in %RGC were obtained during pseudo-backcrossing, whereas rigorous foreground marker-assisted selection tended to reduce linkage drag during pseudo-backcrossing. The evaluation of new traits in selected pseudo-BC3F3BILs indicated significant improvements in resistance to BB, BL, BPH and Sub compared with PinK3, as well as significant improvements in grain yield (21-68%) over the donors, although yield was 7-26% lower than in 'PinK3'. All pyramided lines were aromatic and exhibited improved starch profiles, rendering them suitable for industrial food applications. Results show that our new pyramiding platform, which is based on marker-assisted pseudo-backcrossing, can fix five target genes and three QTLs into a high-yielding pseudo-recurrent background within seven breeding cycles in four years. This multiple pseudo-backcrossing platform decreases the time required to generate new rice varieties exhibiting complex, durable resistance to biotic and abiotic stresses in backgrounds with desirable qualities.
    Full-text · Article · Dec 2015 · Rice
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    • "QTL mapping located qBph3 to the short arm of chromosome 6 between RM589 and RM588 based on KDML105 × Rathu Heenati (Jairin et al. 2009) and PTB33 × RD6 (Jairin et al. 2007) crossings. Other BPH resistance QTLs have also been reported, including Bph17 on chromosome 4 (Sun et al. 2005), Bph4 on chromosome 6 (Kawaguchi et al. 2001; Sun et al. 2006) and Bph18 on chromosome 12 (Jena et al. 2006). In addition to resistance QTLs, a putative sesquiterpene synthase (TPS) gene (Os04g27430) was identified using SFP mapping with isogenic lines derived from the backcross of RH and KDML105 (Kamolsukyunyong et al. 2013). "

    Full-text · Dataset · Feb 2015
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    • "Recently, many additional major genes and QTLs of BPH resistance have been identified and mapped on rice chromosomes by marker-assisted method (Table 2 and 3). For more recent progress and detailed information on these BPH resistance genes/QTLs, see Jena et al. 2006, Chen et al. 2006, Jairin et al. 2007, Rahman et al. 2009, Brar et al. 2009, Myint et al. 2012. "

    Full-text · Article · Feb 2015
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