A fluorescence endomicroscopy for in vivo microscopy of the upper-and the lower GI tract

University of Vic, Vic, Catalonia, Spain
Gastrointestinal Endoscopy (Impact Factor: 5.37). 12/2005; 62(5):686-95. DOI: 10.1016/j.gie.2005.05.021
Source: PubMed


This report describes the development and the clinical evaluation of a novel confocal endomicroscope for obtaining fluorescence images of cellular morphology of the mucosae of the upper- and the lower-GI tract in vivo. The work assessed the feasibility of performing in vivo microscopy at endoscopic examination and evaluated fluorescence imaging protocols.
Images were collected in real time by using two prototype endoscope configurations, featuring slightly different miniaturized fiber-optic confocal microscopes, fitted integrally into the tips of conventional endoscopes. Confocal scanning was performed at 488 nm illumination for excitation of exogenously applied fluorophores (topical acriflavine and intravenous fluorescein). The images were compared with conventional histology of biopsy specimens and the findings of white-light endoscopy.
Confocal endomicroscopy enabled imaging of cellular and subcellular structures (i.e., nuclei) of the GI tract. The crypts of the colonic mucosa, the villi of the terminal ileum and duodenum, the gastric pits of the stomach, and the squamous epithelium of the distal esophagus could be clearly visualized. Acriflavine strongly contrasted the cell nuclei of the surface epithelium, including the absorptive epithelial cells and the mucous secreting goblet cells. Fluorescein stained the extracellular matrix of the surface epithelium and also the subepithelial layers of the lamina propria. Images at increasing depth beneath the epithelium showed the mucosal capillary network. The findings correlated with the histology of biopsy specimens.
The development of a fluorescence confocal endomicroscope makes it practical to examine the upper- and the lower-GI mucosa in cellular detail during otherwise routine endoscopic examination. The results represent a major technical advance in the development of this new optical imaging modality for the in vivo examination of GI tissue.

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    • "Currently, there are two types of CLE: (1) endoscopic-based or integrated confocal laser endomicroscope (eCLE or iCLE, Pentax, Tokyo, Japan) and (2) probe-based confocal laser endomicroscope (pCLE, Mauna Kea Technologies, Paris, France) [10] [11]. From various studies, CLE can provide real-time histology [12] [13] [14] [15] [16] [17] [18] [19] and possibly dynamic cellular changing in gastrointestinal disease [20] [21]. Even though, the learning curve study in CLE is still limited. "

    Full-text · Article · May 2015
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    • "Confocal laser endomicroscopy (CLE) is an emerging technique that can provide real-time images of the gastrointestinal epithelium at the subcellular level in vivo[5] [6]. The technique can help detect the disease at an early stage and reduce the biopsy rate,[7] for an instant classification. "
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    ABSTRACT: Background Confocal laser endomicroscopy (CLE) can provide in vivo subcellular resolution images of esophageal lesions. However, the learning curve in interpreting CLE images of precancerous or early-stage esophageal squamous cancer is unknown. The goal of this study is to evaluate the diagnostic accuracy and inter-observer agreement for differentiating esophageal lesions in CLE images among experienced and inexperienced observers and to assess the learning curve. Method After a short training, 8 experienced and 14 inexperienced endoscopists evaluated in sequence 4 sets of high-quality CLE images. Their diagnoses were corrected and discussed after each set. For each image, the diagnostic results, confidence in diagnosis, quality and time to evaluate were recorded. Results Overall, diagnostic accuracy was greater for the second, third, fourth set of images as compared with the initial set (odds ratio [OR] 2.01, 95% CI 1.22–3.31; 7.95, 3.74–16.87; and 6.45, 3.14–13.27), respectively, with no difference between the third and fourth sets in accuracy (p = 0.67). Previous experience affected the diagnostic accuracy only in the first set of images (OR 3.70, 1.87–7.29, p<0.001). Inter-observer agreement was higher for experienced than inexperienced endoscopists (0.732 vs. 0.666, p<0.01) Conclusion CLE is a promising technology that can be quickly learned after a short training period; previous experience is associated with diagnostic accuracy only at the initial stage of learning.
    Full-text · Article · Jun 2014 · PLoS ONE
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    • "Mucin containing goblet cells remain dark, as does neoplastic tissue.3,4 This contrast effect is present 10 minutes after administration and lasts up to 45 minutes.5 Fluorescein has long been used for ophthalmologic indications and its safety has recently been confirmed for use as a contrast agent in the gastrointestinal tract.6 "
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    ABSTRACT: Probe-based confocal microscopy (pCLE) is actively being investigated for applications in the esophagus and stomach. The use of pCLE allows real-time in vivo microscopy to evaluate the microarchitecture of the mucosal epithelium. pCLE appears to be particularly useful in identifying mucosal dysplasia and early malignancies that cannot be clearly distinguished using high-definition white light endoscopy, chromoendoscopy, or magnification endoscopy. In addition, the ability to detect dysplastic tissue in real-time may shift the current screening practice from random biopsy to targeted biopsy of esophageal and gastric cancers and their precursor lesions. We will review the use of pCLE for detection and surveillance of upper gastrointestinal early luminal malignancy.
    Full-text · Article · Sep 2013 · Clinical Endoscopy
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