Logic of the Yeast Metabolic Cycle: Temporal Compartmentalization of Cellular Processes

Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, L3.124, Dallas, TX 75390, USA.
Science (Impact Factor: 33.61). 12/2005; 310(5751):1152-8. DOI: 10.1126/science.1120499
Source: PubMed


Budding yeast grown under continuous, nutrient-limited conditions exhibit robust, highly periodic cycles in the form of respiratory
bursts. Microarray studies reveal that over half of the yeast genome is expressed periodically during these metabolic cycles.
Genes encoding proteins having a common function exhibit similar temporal expression patterns, and genes specifying functions
associated with energy and metabolism tend to be expressed with exceptionally robust periodicity. Essential cellular and metabolic
events occur in synchrony with the metabolic cycle, demonstrating that key processes in a simple eukaryotic cell are compartmentalized
in time.

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Available from: Andrzej Kudlicki, Jan 22, 2014
    • "Bursts of superoxide and hydrogen peroxide activate cell signalling pathways that trigger the transition from G0 to G1[27,28]. In yeast, oxygen consumption and related metabolism are the lowest during the phases of DNA replication and mitosis[29]. It remains to be clarified whether redox regulation of cell proliferation in the SAM are mechanistically similar to those observed in other eukaryotes. "
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    • "On the basis of their work with the distiller's strain IFO 0233, Klevecz and colleagues first suggested that the observed gating between YMC and CDC might be functionally important for the temporal separation of CDC events (i.e., DNA replication) that are incompatible with YMC events (i.e., HOC and aerobic respiration, which could lead to oxidative damage of DNA; Klevecz et al., 2004). This was confirmed by subsequent work with a different laboratory strain, CEN.PK, which showed that DNA replication occurred toward the end of HOC as pO 2 levels rise (Tu et al., 2005; Chen et al., 2007). The authors classified the end of HOC (when yeast oxygen consumption is still high) as a " reductive-building " phase, based on clustering of gene expression microarrays. "
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