Rapid neurotransmitter uncaging in spatially defined patterns. Nat Methods

Department of Physics, Princeton University, Princeton, New Jersey, United States
Nature Methods (Impact Factor: 32.07). 12/2005; 2(11):837-43. DOI: 10.1038/nmeth793
Source: PubMed


Light-sensitive 'caged' molecules provide a means of rapidly and noninvasively manipulating biochemical signals with submicron spatial resolution. Here we describe a new optical system for rapid uncaging in arbitrary patterns to emulate complex neural activity. This system uses TeO(2) acousto-optical deflectors to steer an ultraviolet beam rapidly and can uncage at over 20,000 locations per second. The uncaging beam is projected into the focal plane of a two-photon microscope, allowing us to combine patterned uncaging with imaging and electrophysiology. By photolyzing caged neurotransmitter in brain slices we can generate precise, complex activity patterns for dendritic integration. The method can also be used to activate many presynaptic neurons at once. Patterned uncaging opens new vistas in the study of signal integration and plasticity in neuronal circuits and other biological systems.

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Available from: Shy Shoham, Dec 08, 2015
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    • "The use of OAM for probing can lead to microscopic imaging with a spatial resolution that is higher than the Rayleigh limit Tamburini et al. (2006) and, when OAM fields are used in combination with suitable fluorescence methods (e.g., the stimulated emission depletion), they enable new methods of far-field microscopy with theoretically unlimited resolution Harke et al. (2006); Hell (2009). Optimal spatially structured light beams have also been considered as tools to cage/uncage specific molecules for accurate and rapid biological imaging Shoham et al. (2005). Some of these approaches may have relevant applications in the imaging of biological tissues, e.g. for diagnostic or research purposes. "

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