NO signaling in ARE-mediated gene expression

ArticleinMethods in Enzymology 396:341-9 · February 2005with1 Read
Impact Factor: 2.09 · DOI: 10.1016/S0076-6879(05)96028-X · Source: PubMed


    Nitric oxide (NO) and peroxynitrite, which serve as cell signal molecules, activate the antioxidant response element (ARE) for the induction of phase II antioxidant enzymes as an adaptive response. The reactive nitrogen species plays an essential role in Nrf2 activation and Nrf2 binding to the ARE present in the target genes. In this chapter, we describe the system by which the NO signaling pathway regulates ARE-mediated gene expression, which includes immunochemical assessment and gel shift analysis of Nrf2 activation.