[Expression of lysophosphatidic acid receptor in human ovarian cancer cell lines 3AO, SKOV3, OVCAR3 and its significance].
To investigate the expressions of lysophosphatidic acid (LPA) receptors at both mRNA and protein levels and their biological significance in different human ovarian cancer cell lines.
Reverse transcription-polymerase chain reaction (RT-PCR), streptovidin peroxidase-conjugated (SP) immunohistochemical assay and Western blotting were employed to measure the expression levels of LPA(1), LPA(2) and LPA(3) mRNA and LPA(2) and LPA(3) protein in cultured human ovarian cancer cell lines 3AO.SKOV3, and OVCAR3.
All the ovarian cancer cell lines expressed LPA(1), LPA(2) and LPA(3) mRNAs and LPA(2) and LPA(3) proteins.
LPA(1), LPA(2) and LPA(3) expressions occur extensively in human epithelial ovarian cancer cell lines, and LPA(2) and LPA(3) may be involved in the development and progression of human ovarian cancer.
Available from: Olaposi Idowu Omotuyi
- "We seek to answer a single question here, what combination of O. Omotuyi et al., Descriptor-based Fitting of Lysophosphatidic Acid Receptor 3 ... molecular predictors would numerically and accurately predict the experimental antagonist activities of LPA 3 inhibitors? When answered, the mathematical relationship derived from the descriptors will enable screening of chemical databases for compounds exhibiting LPA 3 antagonism required for the treatment of diseased conditions such as ovarian cancer  and neuropathic pain  with LPA 3 etiology. "
Available from: wsu.edu
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