Article

New Genotype of Avian Influenza H5N1 Viruses Isolated from Tree Sparrows in China

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071 China.
Journal of Virology (Impact Factor: 4.44). 01/2006; 79(24):15460-6. DOI: 10.1128/JVI.79.24.15460-15466.2005
Source: PubMed

ABSTRACT

The 2004 outbreaks of highly pathogenic avian influenza H5N1 disease in China led to a great poultry loss and society attention.
A survey of avian influenza viruses was conducted on tree sparrows (Passer montanus) collected in China in 2004. Four viruses were isolated from free-living tree sparrows. The results of the whole-genome analysis
indicated that an H5N1 virus with a new genotype is circulating among tree sparrows. The hemagglutinin and neuraminidase genes
of the new genotype were derived from Gs/Gd/96-like viruses and the nuclear protein gene descended from the 2001 genotype
A H5N1 viruses, while the other inner genes originated from an unknown influenza virus. In experimental infection, all four
viruses were highly pathogenic to chickens but not pathogenic to ducks or mice. The four tree sparrow viruses were different
from the 2003 tree sparrow strain (genotype Z) in Hong Kong. The results suggested that H5N1 viruses might be distributed
widely in tree sparrows.

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    • "Influenza viruses have a relatively high mutation rate that is characteristic of RNA viruses. The segmentation of its genome facilitates genetic recombination by segment reassortment in hosts infected with two different influenza viruses at the same time (Kou et al., 2005; WHO, 2005). A previously non contagious strain may then be able to pass between humans, one of several possible paths to a pandemic. "
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    DESCRIPTION: Present study has been carried out with a vision to detect the possible reason i.e. antigenic drift or mutation in viral HA and NA gene of viral isolate of A/Duck/Tripura/103597/2008(H5N1) being a escape mutant since it was reisolated from a bird which was vaccinated with inactivated virus. Both HA and NA whole gene were amplified with gene specific primer specific for HA and NA gene. The amplified HA and NA gene were then cloned in T/A vector as this makes possible to clone PCR product directly into the linearized cloning vector with single 3‘ –T overhangs. The recombinants were then selected on the basis of blue/white colony selection. The HA and NA gene were then amplified and analyzed for the change in HA and NA gene by restriction enzyme profiling pattern using some randomly selected enzymes. The HA Gene was analysed for KpnI, SacI, PstI, EcoRI and XbaI and NA gene was analyzed for BamHI and NcoI restriction pattern. The RE profiling was done thrice to confirm any variation in the cut sites. From the results, no variation/changes were observed in both HA and NA gene from the original predicted RE sites.
    Full-text · Research · Sep 2015
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    • "Influenza viruses have a relatively high mutation rate that is characteristic of RNA viruses. The segmentation of its genome facilitates genetic recombination by segment reassortment in hosts infected with two different influenza viruses at the same time (Kou et al., 2005; WHO, 2005). A previously non contagious strain may then be able to pass between humans, one of several possible paths to a pandemic. "
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    DESCRIPTION: The origin of this study started with the history that a H5N1 virus (A/Duck/Tripura/103597/2008(H5N1) which was formalin inactivated and ensured for inactivation by passaging thrice in embryonated eggs when vaccinated into a group of birds, one exhibited clinical symptoms of infection. The virus was reisolated from the bird and kept in repository. Since point mutation in surface proteins (antigenic drift) may result in new and immunologically different strains of influenza viruses that are responsible for influenza outbreaks and epidemics (Loeffelholz, 2011). This work is planned to infer any changes or mutation or antigenic drift in the HA and NA genes of the reisolated virus, as these are major antigenic determinants and are responsible for infectivity in birds and targets of immune surveillance and generators of neutralizing antibodies. Thus, the present study is designed to clone and to check for any change in restriction enzyme profile pattern of HA and NA gene of reisolated H5N1 virus (from vaccine experiment) from the original virus.
    Full-text · Research · May 2015
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    • "Our studies did not quantify very low levels of virus RNA in the swabs by real-time PCR which was not used in the analyses; moreover, the quantitative results for virus infectivity shown in Figure 1 could not be applied to the low titres of viable virus propagated from swabs from crows since virus was only detected after a blind passage (Table 2). It is known that crows are susceptible to H5N1 avian influenza virus infection [25,41-50], but, to our knowledge, no experimental infection has been reported describing the levels of virus shedding and transmission from H5N1 infected crows to naïve contact crows or chickens. Evidence for widespread prevalence of H9N2 viruses in house sparrows and crows is only limited [14,16], but six H9N2 viruses collected from sparrows in 2005 and 2006 have been described, and the HA genes have been characterised (Zhu,W. "
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    ABSTRACT: Genetic changes in avian influenza viruses influence their infectivity, virulence and transmission. Recently we identified a novel genotype of H9N2 viruses in widespread circulation in poultry in Pakistan that contained polymerases (PB2, PB1 and PA) and non-structural (NS) gene segments identical to highly pathogenic H7N3 viruses. Here, we investigated the potential of these viruses to cause disease and assessed the transmission capability of the virus within and between poultry and wild terrestrial avian species. Groups of broilers, layers, jungle fowl, quail, sparrows or crows were infected with a representative strain (A/chicken/UDL-01/08) of this H9N2 virus and then mixed with naive birds of the same breed or species, or different species to examine transmission. With the exception of crows, all directly inoculated and contact birds showed clinical signs, varying in severity with quail showing the most pronounced clinical signs. Virus shedding was detected in all infected birds, with quail showing the greatest levels of virus secretion, but only very low levels of virus were found in directly infected crow samples. Efficient virus intra-species transmission was observed within each group with the exception of crows in which no evidence of transmission was seen. Interspecies transmission was examined between chickens and sparrows and vice versa and efficient transmission was seen in either direction. These results highlight the ease of spread of this group of H9N2 viruses between domesticated poultry and sparrows and show that sparrows need to be considered as a high risk species for transmitting H9N2 viruses between premises.
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