Intein-mediated in vitro synthesis of lipidated Ras proteins

Chair of Chemical Biology, Technische Universität Dortmund, Dortmund, North Rhine-Westphalia, Germany
Chemical Communications (Impact Factor: 6.83). 02/2006; DOI: 10.1039/b511736d
Source: PubMed


Fully functional lipid-modified Ras proteins suitable for the study of Ras-membrane interactions and embodying exclusively native amide bonds can be synthesized in preparative amounts by means of Expressed Protein Ligation.

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