Identification of Basogranulin (BB1) as a Novel Immunohistochemical Marker of Basophils in Normal Bone Marrow and Patients With Myeloproliferative Disorders

Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.
American Journal of Clinical Pathology (Impact Factor: 2.51). 03/2006; 125(2):273-81. DOI: 10.1309/M9FQ-MQGF-6616-7N2X
Source: PubMed


In myeloproliferative disorders (MPDs), basophils typically increase in number in the bone marrow (BM) and blood. In chronic myeloid leukemia (CML), basophilia is a diagnostic and prognostic variable. However, no reliable approach for routine detection and enumeration of basophils in BM sections is available. We applied the antibasogranulin antibody BB1 on paraffin-embedded BM sections in 21 control samples (normal BM), 45 patients with CML, 9 with chronic idiopathic myelofibrosis, 11 with polycythemia vera, 19 with essential thrombocythemia, and 7 with indolent systemic mastocytosis. As assessed by immunostaining of serial BM sections, BB1+ cells coexpressed myeloperoxidase, histidine decarboxylase, and leukosialin but did not express B- or T-cell-restricted antigens. BB1+ BM cells were found to be highly elevated in patients with CML compared with normal BM or other MPDs, with maximum counts found in accelerated phase CML (median, 160 cells/mm(2)). In summary, BB1 (basogranulin) is a new immunohistochemical basophil marker that should allow quantification of basophils in CML at diagnosis and during therapy.

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    • "In 16 patients with CP CML, 14 with AP, 6 with BP, and 5 with normal BM, expression of HGF was analyzed by IHC on serial sections (2 μm) prepared from formalin-fixed, paraffin-embedded, BM specimens. IHC was performed by the indirect immunoperoxidase staining technique as described [29] using a polyclonal anti-HGF antibody (work dilution, 1:50) and the anti-basophil mAb BB1 (1:300). After incubation (overnight) slides were washed, incubated with biotinylated second step goat anti-rabbit or horse anti-mouse antibodies (30 minutes), washed, and stained using 3-amino-9-ethyl-carbazole (Sigma-Aldrich). "
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    • "So far, only very few immunohistochemical markers sufficient for the evaluation of basophils and eosinophils in BM sections are available. For basophil evaluation and counting, 2D7 and BB1 (basogranulin) are recommended antigens (Figure 1E) [23,24] while eosinophils can be visualized using an antibody against eosinophil major basic protein (EMBP) (Figure 1F). Whereas a slight or moderate increase of eosinophils is often seen in reactive and neoplastic disease states, constant basophilia is uncommon in reactive states and thus regarded as a potential indicator for the presence of a myeloid neoplasm. "
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