Detection of rat parvovirus type 1 and rat minute virus type 1 by polymerase chain reaction

Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, MO 65211, USA.
Laboratory Animals (Impact Factor: 1.12). 02/2006; 40(1):63-9. DOI: 10.1258/002367706775404408
Source: PubMed


Two newly recognized parvovirus species, rat parvovirus 1 (RPV-1) and rat minute virus 1 (RMV-1), were recently identified in naturally infected rats. In this study, two polymerase chain reaction (PCR) assays were developed to specifically detect RPV-1 and RMV-1. The RPV-1 PCR assay amplified the expected 487-bp deoxyribonucleic acid (DNA) fragment only in the presence of RPV-1 DNA; the RMV-1 PCR assay amplified the expected 843-bp product only from RMV-1 DNA, not from other rodent parvoviruses. The RPV-1 and the RMV-1 PCR assays detected approximately 18 and 70 copies of DNA template, respectively. These two PCR assays were shown to be sensitive, specific and rapid methods for detecting RPV-1 and RMV-1 infections in rats. These assays may also be valuable for evaluation of biological specimens for parvovirus contamination.

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Available from: Beth Ann Bauer, Feb 25, 2014
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    • "Little is known about the effects of the other MPV strain variants (MPV-2 and -3) as these were only recently discovered and have not yet been cultivated in cell culture (Besselsen et al. 2006). RPV-1, RMV-1, and RPV/UT also target lymphoid tissues in vivo (Ball-Goodrich et al. 1998; Ueno et al. 1996, 1997; Wan et al. 2006), although there has been little research to determine their specific effects on immunologic responses. RPV-1 delayed onset of clinical signs and leukemia in rats inoculated with large granular lymphocytes (i.e., Fischer rat leukemia cells) (Ball- Goodrich et al. 1998). "
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