Resistoflavine, cytotoxic compound from a marine actinomycete, Streptomyces chibaensis AUBN1/7

Andhra University, Vizag, Andhra Pradesh, India
Microbiological Research (Impact Factor: 2.56). 02/2007; 162(4):322-7. DOI: 10.1016/j.micres.2006.01.012
Source: PubMed


In our systematic screening programme for marine actinomycetes, a bioactive Streptomycete was isolated from marine sediment samples of Bay of Bengal, India. The taxonomic studies indicated that the isolate belongs to Streptomyces chibaensis and it was designated as S. chibaensis AUBN1/7. The isolate yielded a cytotoxic compound. It was obtained by solvent extraction followed by the chromatographic purification. Based on the spectral data of the pure compound, it was identified as quinone-related antibiotic, resistoflavine (1). It showed a potent cytotoxic activity against cell lines viz. HMO2 (Gastric adenocarcinoma) and HePG2 (Hepatic carcinoma) in vitro and also exhibited weak antibacterial activities against Gram-positive and Gram-negative bacteria.

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    • "present in an additional two extracts (1477E and 1494E), which are also present in Cluster A. The identity of each compound was verified by (À)-FTICR MS and analysis of NMR spectra (Figure S1). All three of these compounds have reported cytotoxicity against mammalian cells (Martin et al., 2002; Gorajana et al., 2007; Vijayabharathi et al., 2011). Furthermore, resistomycin has recently been shown to bind DNA by fluorescence competition experiments and circular dichroism studies (Vijayabharathi et al., 2012), and to prevent transcription by inhibition of RNA polymerase (Arora, 1985). "
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