Monocytes-macrophages are a potential target in human infection with West Nile virus through blood transfusion
Laboratory of Molecular Virology (LMV), Division of Emerging Transfusion Transmitted Diseases (DETTD), Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), Bethesda, MD 20892, USA. Transfusion
(Impact Factor: 3.23).
05/2006; 46(4):659-67. DOI: 10.1111/j.1537-2995.2006.00769.x
West Nile virus (WNV) transmission by transfusion was documented in 2002. Approximately 80 percent of WNV infections are asymptomatic and 1 percent develop severe neurological illness. In animals, Langerhans-dendritic cells support initial viral replication, followed by replication in lymphoid tissues and dissemination to organs and possibly to the CNS. The cellular tropism of WNV infection after transfusion and the particular human blood cells that sustain viral replication remain largely unknown. Whether primary monocyte-derived macrophages (MDMs) support WNV infection-replication and produce infectious virions, with an in vitro system, was investigated.
Elutriated monocytes (CD33+/CD14+) from suitable blood donors were cultured in the presence of macrophage-colony-stimulating factor, infected with WNV-NY99 at different time points, washed, and cultivated for up to 47 days. Supernatants were tested for WNV replication by TaqMan reverse transcription-polymerase chain reaction (RT-PCR), with primers for the envelope and/or 3'NC regions, and by cDNA-PCR to detect WNV minus-strand RNA and for the presence of functional virions by infectivity assays in Vero cells.
RT-PCR TaqMan of supernatants demonstrated productive infection of MDMs. Viral load reached 2 to 5 log above baseline in 3 to 6 days and then declined, with detectable viral replication persisting for up to 47 days. WNV minus-strand RNA was detected in Day 4 cultures, indicating active viral replication. Infected MDM cultures showed no cytopathic changes. Supernatants that were TaqMan-positive for the presence of WNV-infected Vero cells and produced cytopathic effects within 3 to 5 days of culture.
The susceptibility of monocytes-macrophages to productive infection in vitro is compatible with a potential role in initial WNV replication and propagation after transmission by transfusion.
Available from: Kiran Kundu
- "Apart from the central depots in secondary lymphoid organs, macrophages reside virtually in all tissues . It has been reported in several studies that macrophages can be effectively infected by arthropod-borne flaviviruses    . Upon viral sensing or chemotactic signals released by other infected cells, macrophages produce cyto/chemokines and other inflammatory mediators which serve as the activators of adaptive immune system, leading to the ultimate clearance of the virus from the body . "
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ABSTRACT: a b s t r a c t Viruses have evolved various mechanisms to subvert the host's immune system and one of them is pre-venting the infected cells from sending out chemotactic signals to activate the adaptive immune response. Japanese encephalitis virus (JEV) is a neuropathologic flavivirus that is responsible for signifi-cant number of child mortalities in various parts of South-East Asia. In this study we show that JEV mod-ulates suppressors of cytokine signaling (SOCS)1 and 3 expression in macrophages to bring about changes in the JAK–STAT signaling cascade, so as to inhibit proinflammatory cyto/chemokine release. Using real time PCR, immunoblotting and immunofluorescent staining, we show that the expression of type 1 inter-ferons and intracellular expression of viral genes are also affected over time. Also, following the initial activation of SOCS1 and 3, there is production of interferon-inducible anti-viral proteins in the cells which may be responsible for inhibiting viral replication. However, even at later time points, viral genes were still detected from the macrophages, albeit at lesser quantities, than earlier time points, indicative of intracellular persistence of the virus in a latent form. On knocking down SOCS1 and SOCS3 we found a significant decrease in viral gene expression at an early time point, indicating the dysregulation of the signaling cascade leading to increased production of interferon-inducible anti-viral proteins. Taken together, our study provides an insight into the role of JEV infection in modulating the JAK–STAT pathway with the help of SOCS leading to the generation of an antiviral innate immune response.
Available from: Takashi Onodera
- "The isolation of causative virus from field materials is an important task for reference laboratories and a sensitive assay method is desired even though several diagnostic protocols have been established. Accumulating information indicate mononuclear phagocytes (monocyties, macrophages, and dendritic cells) are sensitive enough not only for WNV (Mather et al., 2003; Rios et al., 2006) but also for Dengue virus (Wang et al., 2002; Blackley et al., 2007; Kou et al., 2008), for human immunodeficiency virus (Cassol et al., 2006; Shen et al., 2011), for Crimean Congo hemorrhagic fever virus (Connolly et al., 2009), for Visna/Maedi virus (Narayan et al., 1982; Crespo et al., 2011), for Tanapox virus (Nazarian et al., 2007), for Measles virus (Lemon et al., 2011), and for Aujeszky disease virus (Kadoi, 2002). We have succeeded to establish a horse monocytic cell line (HsMo) and a mouse monocyrtic cell line (MsMo) and their susceptibility to WNV was examined in comparison with Vero cells. "
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ABSTRACT: Two monocytic cell lines, temporary named HsMo (horse monocyte) and MsMo (mouse monocyte) were established from blood samples of horse and Balb/C mouse according to the culture method previously informed (Kadoi, 2011). Tests were performed to certify the viral susceptibility of these cells in comparison with that of Vero cells for West Nile virus (WNV). Two strains of WNV adapted to grow in Vero cells were titrated for infectivity in the monocytic cells and Vero cells in a routine microplate assay system. The highest titer was obtained in the assay system with HsMo cells followed by the system with MsMo cells. These monocytic cells were sensitive enough for detecting WNV, however, WNV infected monocytes produced a strong acidic substance in which the acidity is much higher grade than that produced in Vero cells. In microplate culture the color of medium changes from redden neutral color to yellow indicated by phenol red dye included in cell culture medium, in parallel with CPE occurrence. This is a color test for WNV assay in monocytic cells Keywords: Horse monocytes, mouse monocytes, WNV susceptibility. INTRDUCTION The invasion of West Nile virus (WNV) became a worldwide serious problem since the causative pathogenic virus has readily spread in many countries, Africa, the Middle East, Europe, USA, USSR, and Asia. (Dauphin et al., 2004; Zeller and Schuffenecker, 2004; Hayes et al., 2005). WNV is an arbovirus transmitted by various mosquito species (Anderson et al., 1999). This is the main reason for the elimination of the disease once introduced to virgin area. Several animal species are known to be susceptible hosts (Lanciotti et al., 1999; Weingartle et al., 2004) and birds are suspected to be amplifying host (Anderson et al., 1999; Kramer and Bernard, 2001; McLean et al., 2001; Weingartle et al., 2004). The virus was isolated from samples of humans followed by horses in the beginning of the history. However, it was now understood that the virus is widely distributed in animal world since the virus was isolated not only clinically affected animals, but was also isolated from asymptomatic animals as camel and grass mouse,
Available from: Thomas Welte
- "In vivo depletion of macrophages had an exacerbating effect on the course of the infection by an attenuated WNV strain, exhibited by higher and extended viremia and accelerated development of encephalitis and death (Ben-Nathan et al., 1996). Nevertheless, the susceptibility of monocytes /macrophages to productive WNV infection in vitro (Cardosa et al., 1983) is also compatible with a potential role in initial WNV replication and propagation in humans (Rios et al., 2006). In support of this notion, silencing early viral replication in macrophages in mice seems to effectively suppress WNV induced encephalitis (Ye et al., 2003). "
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