High-Throughput RNA Interference in Functional Genomics

Department Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Fabeckstr. 60-62, 14195 Berlin, Germany.
Handbook of experimental pharmacology 02/2006; 173(173):97-104. DOI: 10.1007/3-540-27262-3_5
Source: PubMed


RNA interference (RNAi) refers to post-transcriptional silencing of gene expression as a result of the introduction of double-stranded RNA into cells. The application of RNAi in experimental systems has significantly accelerated elucidation of gene functions. In order to facilitate large-scale functional genomics studies using RNAi, several high-throughput approaches have been developed based on microarray or microwell assays. The recent establishment of large libraries of RNAi reagents combined with a variety of detection assays has further improved the performance of functional genome-wide screens in mammalian cells.

1 Follower
10 Reads
  • [Show abstract] [Hide abstract]
    ABSTRACT: NOx-catalyzed oxidation of methane without any solid catalyst has been investigated, hydrogen selectivity of 27% has been obtained with an overall methane conversion of 34% and a free O2 concentration of 1.7% at 700 °C.
    No preview · Article · Jan 2004 · Studies in surface science and catalysis
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A modern concept for the development of novel antiparasitic drugs is the combination of bioinformatics and chemoinformatics approaches. This covers, for example, the identification of target proteins serving as molecular points of attack for parasiticides--the idea is that, owing to some essential role, inhibition of a target protein should eradicate the parasite. To prevent toxicity problems for vertebrate host organisms, it is advantageous that these proteins show significant differences from their vertebrate counterparts. In the present work, we identified potential target proteins in parasitic nematodes (Ascaris suum, Brugia malayi, and Haemonchus contortus) and arthropods (Boophilus microplus and Rhipicephalus appendiculatus) using bioinformatic sequence comparison methods on expressed sequence tags. Interesting target proteins (e.g., S-adenosyl-l-methionine synthetase) were characterized in detail by subjecting them to in-depth bioinformatic analysis. S-Adenosyl-l-methionine synthetase was also used to elucidate chemoinformatics approaches like homology modeling and docking, which represent appropriate methods for generating valuable data for the development of new drug candidates.
    Full-text · Article · Feb 2007 · Genomics
  • [Show abstract] [Hide abstract]
    ABSTRACT: Neurite outgrowth involves various molecular mechanisms generating complex brain connections. These mechanisms have been linked to plasticity and learning and are thought to be deregulated in neuropsychiatric diseases. The transcription factor REST/NRSF regulates a subset of genes encoding neurite outgrowth molecules. We demonstrate here the downregulation of Rest/Nrsf expression in a mouse neuroblastoma cell line. This downregulation induced a clear increase in neurite length. Quantitative polymerase chain reaction showed deregulation of the candidate genes L1cam, Elmo2, Ulip1 and Ulip2. These genes are bona fide candidates known to be involved in dendrite and axonal outgrowth. This approach could be adapted to high-throughput techniques for determination of the mammalian neurite outgrowth gene repertoire.
    No preview · Article · Apr 2007 · Neuroreport
Show more