Norepinephrine Modulates the Inflammatory and Proliferative Phases of Wound Healing

Burn and Shock Trauma Institute, Loyola University Medical Center, Maywood, IL 60153, USA.
The Journal of trauma (Impact Factor: 2.96). 05/2006; 60(4):736-44. DOI: 10.1097/
Source: PubMed


Injury results in the massive release of norepinephrine (NE) into the peripheral circulation. Recent investigations have demonstrated functional adrenoreceptors on the cellular mediators of cutaneous wound healing and NE-induced phenotypic alterations in immune cells have been demonstrated in vitro. Despite this, there is little description of how NE might alter the phases of wound healing in vivo. The purpose of this study was to compare cutaneous wound healing in norepinephrine-intact and norepinephrine-depleted mice.
Norepinephrine-depleted (NED) mice were generated by chemical axotomy with 6-hydroxydopamine and compared with norepinephrine-intact (NEI) animals (n = 6-12 per group, per time point). Using an excisional wound model, neutrophil recruitment was measured by myeloperoxidase assay. Macrophage recruitment and angiogenesis were measured by immunohistochemistry and re-epithelialization was determined histologically. The development of incisional wound disruption strength was determined over time. Finally, macrophage scavenger function was assessed by an in vitro latex bead phagocytosis assay.
Wounds from NEI mice demonstrated greater neutrophil infiltration than NED wounds (24, 72 hours; p < 0.05). Wound macrophage recruitment was initially higher in NEI animals (24 hours, p < 0.05), but was eventually surpassed by that of NED animals (120 hours, p < 0.05). Angiogenesis was decreased while re-epithelialization was accelerated in NEI animals (p < 0.05). Wound disruption strength and macrophage scavenger function were unaltered between NED and NEI mice.
Norepinephrine modulates the inflammatory and proliferative phases of wound healing in a temporally defined, cell-specific manner. By increasing recruitment of innate immune cells and expediting wound closure, norepinephrine appears to play a protective role in defense against infection.

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    • "Initial studies showed that sympathetic denervation accelerates wound contraction and increases myofibroblast density, but impairs re-epithelialization, inflammation, and mast cell accumulation in rat excisional wounds.[5] Subsequently, it was shown that mice depleted of norepinephrine by chemical axotomy with 6-hydroxydopamine exhibited reduced re-epithelialization and infiltration of neutrophils and macrophages, and increased angiogenesis.[6] Recent findings suggest that these effects are mediated by blockade of the β-adrenergic receptors, resulting in aberrant wound healing.[7] "
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