Mantena SK, Katiyar SKGrape seed proanthocyanidins inhibit UV-radiation-induced oxidative stress and activation of MAPK and NF-kappaB signaling in human epidermal keratinocytes. Free Radic Biol Med 40:1603-1614

Department of Dermatology, University of Alabama at Birmingham, Volker Hall 557, 1670 University Boulevard, P.O. Box 202, Birmingham, AL 35294, USA.
Free Radical Biology and Medicine (Impact Factor: 5.74). 06/2006; 40(9):1603-14. DOI: 10.1016/j.freeradbiomed.2005.12.032
Source: PubMed


Solar ultraviolet (UV) radiation-induced oxidative stress has been implicated in various skin diseases. Here, we report the photoprotective effect of grape seed proanthocyanidins (GSPs) on UV-induced oxidative stress and activation of mitogen-activated protein kinase (MAPK) and NF-kappaB signaling pathways using normal human epidermal keratinocytes (NHEK). Treatment of NHEK with GSPs inhibited UVB-induced hydrogen peroxide (H2O2), lipid peroxidation, protein oxidation, and DNA damage in NHEK and scavenged hydroxyl radicals and superoxide anions in a cell-free system. GSPs also inhibited UVB-induced depletion of antioxidant defense components, such as glutathione peroxidase, catalase, superoxide dismutase, and glutathione. As UV-induced oxidative stress mediates activation of MAPK and NF-kappaB signaling pathways, we determined the effects of GSPs on these pathways. Treatment of NHEK with GSPs inhibited UVB-induced phosphorylation of ERK1/2, JNK, and p38 proteins of the MAPK family at the various time points studied. As UV-induced H2O2 plays a major role in activation of MAPK proteins, NHEK were treated with H2O2 with or without GSPs and other known antioxidants, viz. (-)-epigallocatechin-3-gallate, silymarin, ascorbic acid, and N-acetylcysteine. It was observed that H2O2-induced phosphorylation of ERK1/2, JNK, and p38 was decreased by these antioxidants. Under identical conditions, GSPs also inhibited UVB-induced activation of NF-kappaB/p65, which was mediated through inhibition of degradation and activation of IkappaBalpha and IKKalpha, respectively. Together, these results suggest that GSPs could be useful in the attenuation of UV-radiation-induced oxidative stress-mediated skin diseases in human skin.

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Available from: Sudheer Mantena, Dec 05, 2014
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    • "6C–D). Previous study showed that exposure of UVB radiation resulted in the degradation of IκBα protein and subsequent activation and translocation of NF-κB/p65 to the nucleus (Mantena and Katiyar, 2006). To study the inhibitory effect of C3G on UVB-induced degradation of IκBα, we determined the cytoplasmic level of IκBα protein expression. "
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    • "GSP's also inhibit phosphorylation of ERK1/2, JNK, p38 and proteins of MAPK family, as well as UVB-induced activation of NF-B/p65. These results suggest that GSP may attenuate UV-induced oxidative stress in human skin Mantena andKatiyar (2006)Green tea extract a Green tea extract enhances skin photoprotection through anti-inflammatory, antioxidant, and DNA repair mechanisms. In mice stimulated by psoralen and UVA (a quite common psoriasis treatment), orally-administered green tea extract inhibited c-fos and p53 protein accumulation. "
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    • "Mantena and Katiyar [54] attempted to define the photoprotective mechanism of action of GSPs. Researchers observed that in vitro treatment of NHEK with GSPs resulted in the prevention of UVB-induced depletion of antioxidant defense enzymes (GPx, catalase, SOD, and GSH) and H2O2 production. "
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