Article

Lam KS Discovery of novel metabolites from marine actinomycetes. Curr. Opin. Microbiol. 9, 245-251

Department of Microbiology and Anti-infective Discovery, Nereus Pharmaceuticals, Inc., 10480 Wateridge Circle, San Diego, CA 92121, USA.
Current Opinion in Microbiology (Impact Factor: 5.9). 07/2006; 9(3):245-51. DOI: 10.1016/j.mib.2006.03.004
Source: PubMed

ABSTRACT

Recent findings from culture-dependent and culture-independent methods have demonstrated that indigenous marine actinomycetes exist in the oceans and are widely distributed in different marine ecosystems. There is tremendous diversity and novelty among the marine actinomycetes present in marine environments. Progress has been made to isolate novel actinomycetes from samples collected at different marine environments and habitats. These marine actinomycetes produce different types of new secondary metabolites. Many of these metabolites possess biological activities and have the potential to be developed as therapeutic agents. Marine actinomycetes are a prolific but underexploited source for the discovery of novel secondary metabolites.

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    • "Hence researchers are now searching rare ecosystems, instead of normal terrestrial sources, such as marine, mountain and forest ecosystems for actinobacteria for potentially new biosynthetic diversity (Lam, 2006; Berdy, 2012). With this view the present study was attempted to study the antitubercular activity of forest soil Streptomyces sp SFA5. "
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    ABSTRACT: Extracellular pigment from the forest soil Streptomyces sp SFA5 was produced by submerged fermentation using yeast extract malt extract broth. Crude pigment from the medium was extracted using ethyl acetate. Antitubercular activity of the pigment was tested against M. tuberculosis H37Rv by microplate alamar blue assay and luciferase reporter phage assay. The pigment was also tested for inhibitory activity against M. tuberculosis lysine aminotransferase by colorimetric method. In both microplate alamar blue and luciferase reporter phage assay, the crude pigment showed activity against M. tuberculosis H37Rv at 125 and 250 µg/mL concentration, respectively. The M. tuberculosis lysine aminotransferase was inhibited at the IC50 value of 4.5 µg/mL concentration.
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    • "Several actinomycetes, found to be proficient to produce antimicrobial compounds and halotolerant enzymes, have been reported from the coastal solar salterns (Vasavada et al. 2006; Aruljose et al. 2011). Actinomycetes are the most economically and biotechnologically valuable prokaryotes (Lam 2006) On the other hand, a great metabolic diversity and biotechnological potential has been found in halophilic and halotolerant microorganisms. "
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    ABSTRACT: Antagonistic Streptomyces spp. AJ8 was isolated and identified from the Kovalam solar salt works in India. The anti-microbial NRPS cluster gene was characterized by PCR, sequencing and predict the secondary structure analysis. The secondary metabolites will be extracted from different organic solvent extraction and studied the antibacterial, anti-fungal, antiviral and anticancer activities. In vitro antagonistic activity results revealed that, Streptomyces spp. AJ8 was highly antagonistic against Staphylococcus aureus, Aeromonas hydrophila WPD1 and Candida albicans. The genomic level identification revealed that, the strain was confirmed as Streptomyces spp. AJ8 and submitted the NCBI database (KC603899). The NRPS gene was generated a single gene fragment of 781 bp length (KR491940) and the database analysis revealed that, the closely related to Streptomyces spp. SAUK6068 and S. coeruleoprunus NBRC15400. The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition. The antiviral activity results revealed that, the metabolite was significantly (P < 0.001) controlled the killer shrimp virus white spot syndrome virus at the level of 85 %. The metabo-lite also suppressed the L929 fibroblast cancer cells at 35.7 % viability in 1000 µg treatment.
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    • "According to the results of Takahashi et al. (1993), most of the actinobacteria occur within one meter below the ground. Compared to terrestrial soils, the marine sediments have proved to be the best source for the isolation of antagonistic actinobacteria by various workers not only from marine soils and sediments (Vijayakumar et al., 2007Vijayakumar et al., , 2012a–c) but also from salt mining samples (Yang et al., 2008); mangrove environments (Dhanasekaran Remya and Vijayakumar, 2008 ), estuaries, sand dunes and industrially polluted coast soil, and salt marsh soil (Al-Zarban et al., 2002; Kathiresan et al., 2005); coral reefs (Lam, 2006); marine sediments (Olano et al., 2009); salt pan environment (Vijayakumar et al., 2012c); sea anemone (Chen et al., 2009); marine sponge (Gandhimathi et al., 2009); beach soil (Ogunmwonyi et al., 2010); endophytic actinobacteria (Ravikumar et al., 2010); seawater (Reddy et al., 2011); and saltern (Chun et al., 2000). "

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