Periodontopathic bacterial endotoxin-induced tumor necrosis factor α production was inhibited by exercise in mice

Department of Microbiology, Oral Health Science Center, Tokyo Dental College, Chiba, Japan.
FEMS Immunology & Medical Microbiology (Impact Factor: 3.08). 07/2006; 47(2):262-6. DOI: 10.1111/j.1574-695X.2006.00075.x
Source: PubMed


The effects of exercise on serum interleukin-6 and tumor necrosis factor alpha levels were investigated using mice. Five-week-old female BALB/c mice (Th2-biased) and C57BL/6 mice (Th1-biased) were divided into exercise and control groups. The exercise group was exercised in a rotating basket type treadmill for 1 h (5 r.p.m.). Blood was collected and the serum was separated immediately after exercise. The serum interleukin-6 and tumor necrosis factor alpha levels were measured using an Endogen ELISA kit. Exercise significantly increased the serum interleukin-6 level in the two strains of mice (P<0.05 and P<0.01). The tumor necrosis factor alpha level was decreased in the exercise group. Next, periodontopathic bacterial endotoxin (lipopolysaccharide) was administered after exercise, and the effects of exercise on the lipopolysaccharide-induced serum interleukin-6 and tumor necrosis factor alpha levels were investigated. Exercise inhibited lipopolysaccharide-induced tumor necrosis factor alpha production, suggesting it has a defensive action against endotoxin shock.

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Available from: Ryuta Kimizuka, Jan 25, 2015
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    • "Harvested cells were washed with phosphate-buffered saline (PBS; pH 7.2) and suspended in the same buffer. Lipopolysaccharide was prepared by the hot phenol-water method (Westphal & Jann, 1965) and purified as described previously (Kato et al., 2006). Lipopolysaccharide fractions were assessed for purity by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using silver staining. "
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