[Effects of flunarizine on T-type calcium channels in mouse spermatogenic cells].
To investigate the effects of Flunarizine (Flu) on T-type calcium currents (ICaT) in spermatogenic cells.
Ca2+ currents were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp technique and the effects of Flu on ICaT were observed.
Flu of 0.1, 1, 10, 100 micromol/L inhibited ICaT in mouse spermatogenic cells significantly with the K50 value of 0.289 micromol/L (P < 0.05). With the holding potential at -90 mV and stimulating potential at -30 mV, the inhibition rates of Flu were (23.34 +/- 2.76)%, (46.04 +/- 3.52)%, (62.52 +/- 3.70)% and (73.52 +/- 3.12)%, respectively.
Flu has significant inhibitory effects on ICaT in mouse spermatogenic cells, with concentration dependence. Ca(v)3.2 is the main contributor to T-type Ca2+ currents in spermatogenic cells.
Available from: Alberto Darszon
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ABSTRACT: Cell function is importantly regulated by the intracellular concentration of Ca(2+) ([Ca(2+)]i). Sperm development and function are deeply influenced by [Ca(2+)]i which is modulated amongst other ion transporters by plasma membrane Ca(2+) permeable channels. The presence and role of voltage-dependent Ca(2+) channels (CaV) of the T-type (CaV3) in sperm physiology have become a matter of debate in recent years. Though they are functionally present in later stages of development in spermatogenic cells and testicular sperm and their mRNAs and proteins detected from spermatogenic cells to mature mammalian spermatozoa, their currents have not been recorded in mature spermatozoa. This review critically summarizes the evidence for the involvement of CaV3 channels in sperm development and function.
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