Stromal differences in salivary gland tumors of a common histopathogenesis but with different biological behavior: A study with picrosirius red and polarizing microscopy

Department of Oral Pathology and Oral Medicine, The Maurice and Gabriella Goldschleger School of Dental Medicine, Tel Aviv University, Ramat Aviv 69978, Israel.
Acta Histochemica (Impact Factor: 1.71). 11/2006; 108(4):259-64. DOI: 10.1016/j.acthis.2006.05.007
Source: PubMed


Salivary gland neoplasms - pleomorphic adenoma, polymorphous low-grade adenocarcinoma, and adenoid cystic carcinoma - share a common histogenetic trait, but differ markedly in their biological properties. The objective of the study was to assess the polarization colors of picrosirius red-stained stromal collagen fibers in these salivary gland neoplasms to evaluate their possible role in the histopathogenesis of the tumors and to evaluate the potential usefulness of this approach as a diagnostic tool. Ten cases of each tumor type and 10 cases of mucous extravasation phenomenon (control) were examined using picrosirius red staining and polarizing microscopy. In each case, at least 50 thin ( approximately 0.8 microm) and 50 thick (1.6-2.4 microm) collagen fibers were counted and classified as green-yellow or yellow-orange, the mean percentage was calculated and statistical differences analyzed by one-way ANOVA. Results showed a similar thin fiber distribution in all tumor types and controls (82-88% green-yellow, 12-18% yellow-orange, p>0.05). Thick fibers showed a different distribution in polymorphous low-grade adenocarcinoma and adenoid cystic carcinoma (approximately 50% green-yellow) compared to pleomorphic adenoma and mucous extravasation phenomenon (approximately 13% green-yellow) (p=0.001). Thick fiber distribution was similar in polymorphous low-grade adenocarcinoma and adenoid cystic carcinoma (p>0.05). We conclude that with picrosirius red staining and polarizing microscopy, stromal collagen fibers differ significantly in pleomorphic adenoma from those in polymorphous low-grade adenocarcinoma and adenoid cystic carcinoma, but not from mucous extravasation phenomenon. Similarity between polymorphous low-grade adenocarcinoma and adenoid cystic carcinoma may indicate that these tumor types represent a single entity with a broad spectrum of biological behavior.

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Available from: Irit Allon, May 17, 2014
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    • "SCC is primarily composed of malignant epithelial cells and stroma in which they are dispersed [2]. The birefringence of collagen is related to its physical aggregation which could be altered due to action of collagenases, matrix metalloproteinases (MMPs) secreted by tumour cells [2] [3] [4]. In present study, an attempt was made to observe if there is any change in nature of collagen fibres in different grades of oral SCC (OSCC) by determining the ratio of thick to thin fibers as well as their polarizing colours in Picrosirius red stained sections. "
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    • "ACTHIS-50840; No. of Pages 7 B. Buffoli et al. / Acta Histochemica xxx (2014) xxx–xxx 3 Sirius Red staining For Sirius Red staining, the sections were incubated in 1% acid phosphomonolybdic aqueous solution for 5 min and then in 0.1% (w/v) Sirius Red (Direct Red 80, 365548, Sigma–Aldrich, Milan, Italy) in saturated picric acid solution for 60 min, according to Puchler's method (Sweat et al., 1964; Allon et al., 2006) All sections were then analyzed using a light microscope (Olympus BX50, Olympus, Hamburg, Germany) equipped with U-POT polarizer (Olympus, Hamburg, Germany) to analyze the organization of collagen fibers, according to Rodella et al. (2006): under polarized light microscopy, the dense collagen fibers were stained orange-red, whereas the thin collagen fibers appeared green. A qualitative analysis was performed in a blind study by two investigators unaware of the group assignment. "
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    • "Sirius red F3BA dissolved in a saturated picric acid stains collagens type I and III, which can be distinguished with the use of polarized light, and their content evaluated by computer assisted morphometry is in concordance with the results obtained by immunohistochemistry and evaluation of mRNA levels [29,30]. Modified technique with picrosirius red [31] was used as follows: the slides were submerged in 0.2% phosphomolybdic acid, then stained with 0.1% sirius red F3BA in a saturated water solution of picric acid for 90 min, washed in 0.01 N HCl, dehydrated and mounted. "
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