Value of host range, morphological, and genetic characteristics within the Entomophthora muscae species complex

Department of Ecology, The Royal Veterinary and Agricultural University (KVL), Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. <>
Mycological Research (Impact Factor: 2.81). 09/2006; 110(Pt 8):941-50. DOI: 10.1016/j.mycres.2006.06.003
Source: PubMed


Entomopthora muscae sensu lato is a complex of morphologically similar fungal species pathogenic to evolutionarily advanced flies (Cyclorrhapha). To reach an operational species definition and recognition of species within this complex, the values of host range, morphological and genetic characteristics are reconsidered. Within the E. muscae species complex morphological and nuclear characteristics of the primary conidia are taxonomically important. In this study we compared the dimensions and nuclear numbers of the primary conidia of isolates from their original (natural) hosts and after being transferred to alternative hosts (cross-transmission) in order to check the stability of these characteristics. The conidial characteristics change substantially when produced in alternative host species, but their overall range in variability still fit within the traditional morphological species circumscriptions. The phylogenetic analyses of the ITS II and LSU rRNA gene sequences, revealed three distinct lineages within the complex: E. schizophorae, E. muscae and E. syrphi. Within each of these lineages sequence divergence was seen between isolates originating from different host species. Our studies on the physiological host range showed that several isolates were able to infect alternative dipteran species. Musca domestica was a particularly good receptor. The ecological host range of any individual isolate seems, however, to be limited to one host species evidenced by the occurrence of distinct genotypes within each natural infected host species shown by RAPD. The high host specificity of these fungi emphasizes the importance of identifying the host taxon at species level in the recognition of Entomophthora species. We recommend that morphological characteristics of fungal structures and host taxon, together with molecular data, serve as criteria for species determination in future studies on members of the E. muscae complex.

Download full-text


Available from: Jørgen Eilenberg
  • Source
    • "In Diptera, fungi from the genus Entomophthora have over time diverged much as have their host dipterans, by that documenting a long history of co-evolution (Jensen et al., 2009). A certain success in transmission of Entomophthora from its original host to other dipteran hosts was, however obtained by Jensen et al. (2006), although such transmission was accompanied by a less profound sporulation from the new host. "

    Full-text · Article · Jan 2015
  • Source
    • "Thus far, 223 species of Entomophthorales have been documented. Their enormous isolates derived from the carcasses of diverse host species or geographical origins exhibit high variability in terms of genetic character� istics as well as sporulation and infection capacities (Rohel et al., 1997; �ierotzki et al., 2000; Jensen et al., 2006; Keller 2007; �osa�Gómez et al. 2010). Isolates of high conidial production and infectivity formulated or not have been applied for decades in classical and conservation biological control programs against agri� cultural and forest pests (Pell et al., 2010; Hajek and Delalibera 2010). "

    Full-text · Article · Jan 2014 · Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists
  • Source
    • "isolates of soil fungi may have specialized in their function to produce specific organic acids , which may have led to the high variation in RSE of the Mexican isolates . Differences in PCa - and PFe - solubilizing capacity within each fungal genus may also be related to site specific selections and thus genetic variations among fungal isolates ( Jensen et al . 2006 ; Largeteau et al . 2006 ; Gajera and Vakharia 2010 ; Knight et al . 2010 ) . The carbon sources for the growth of the phosphate - solubilizing fungi may have influenced their phosphate - solubilizing activity . In our laboratory tests , the carbon source was D - glucose , an easily assimilated monosaccharide , but one that induces low "
    [Show abstract] [Hide abstract]
    ABSTRACT: Almost 900 fungal isolates were obtained from eight coffee plantations in Colombia and Mexico. Of these, 76 isolates showed activity to solubilize Ca3(PO4)2 (PCa) and FePO4·H2O (PFe), which had been added to agar in a plate test. Generally, PCa was better solubilized than PFe. Colombian isolates were generally somewhat less effective than Mexican isolates. The two most effective isolates from each country with apparent highest PFe, solubilization potential were selected and cultivated in liquid medium containing PFe, which is more prevalent in tropical soils. The pH value, solubilized P in the medium and P uptake in fungal biomass were determined. After 24 days, Cylindrocarpon didymum and C. obtusisporum (both from Colombia) had solubilized 9.9 and 6.4 mg PO4 -P L and took up 8.6 and 11.6 mg P in biomass. Penicillium janthinellum and Paecilomyces marquandii (both from Mexico) solubilized 7.0 and 1.9 mg PO4 -P L and took up 11.3 and 17.3 mg P in biomass. The potential practical use of the four fungal isolates for different strategies in making more P available for coffee growth is discussed.
    Full-text · Article · Feb 2013 · Archives of Agronomy and Soil Science
Show more