Genetic interactions between Drosophila melanogaster menin and Jun/Fos

National Institute of Diabetes and Digestive and Kidney Diseases, Department of Health and Human Services, Bethesda, MD 20892, USA.
Developmental Biology (Impact Factor: 3.55). 11/2006; 298(1):59-70. DOI: 10.1016/j.ydbio.2006.06.013
Source: PubMed


Menin is a tumor suppressor required to prevent multiple endocrine neoplasia in humans. Mammalian menin protein is associated with chromatin modifying complexes and has been shown to bind a number of nuclear proteins, including the transcription factor JunD. Menin shows bidirectional effects acting positively on c-Jun and negatively on JunD. We have produced protein null alleles of Drosophila menin (mnn1) and have over expressed the Mnn1 protein. Flies homozygous for protein-null mnn1 alleles are viable and fertile. Localized over-expression of Mnn1 causes defects in thoracic closure, a phenotype that sometimes results from insufficient Jun activity. We observed complex genetic interactions between mnn1 and jun in different developmental settings. Our data support the idea that one function of menin is to modulate Jun activity in a manner dependent on the cellular context.

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    • "GAL4 targeted RNAi knockdown technology (Brand and Perrimon 1993) was used to create transgenic flies capable of inducible reduction of endogenous Dmel\ELP3 in specific cell and tissue-types of choice. This strategy has been successfully used for functional analysis of numerous genes in Drosophila (Cerrato et al. 2006; Zhu et al. 2007; Rushton et al. 2009). The Dmel\ELP3/RNAi construct was created by selecting a 650 bp RNAi non-conserved target sequence specific for Dmel\ELP3 (Fig. 1a and b) shown by Basic local alignment search tool (BLAST) searches to exhibit non-redundancy within the genome. "
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