Asymptomatic circulation of HEV71 in Norway. Virus Res

Charles University in Prague, Praha, Praha, Czech Republic
Virus Research (Impact Factor: 2.32). 02/2007; 123(1):19-29. DOI: 10.1016/j.virusres.2006.07.015
Source: PubMed


Widespread circulation of human enterovirus 71 was discovered in a prospective study of fecal samples obtained from healthy Norwegian children. Molecular characterization of the virus determined that it belonged to genotype C1. Complete sequencing of this strain, HEV71 804/NO/03, revealed differences in the 5'UTR and polymerase with respect to more pathogenic genotypes that may explain its reduced neurovirulence.

Download full-text


Available from: Elisabet Witsø
    • "Subsequently, SAFV has been found in stool1213141516171819, respiratory[20,21], sewage[22], cerebrospinal fluid, blood and myocardium samples[15], and seems to infect young children[23]. The distribution and associated symptoms of SAFV are still not well described, but SAFV has been reported in both asymptomatic and symptomatic infections, as is also the case for other human picornaviruses such as enteroviruses and pare- choviruses[24,25]. Given the associated symptoms and diabetogenic potential of cardioviruses in rodents, and of related viruses in the picornaviridae family in humans, it is of interest to study the potential prospective association of SAFV with reported symptoms of disease and with development of islet autoimmunity and type 1 diabetes. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Aims: The aim of this study was to investigate the longitudinal plasma metabolic profiles in healthy infants and the potential association with breastfeeding duration and islet autoantibodies predictive of type 1 diabetes. Method: Up to four longitudinal plasma samples from age 3 months from case children who developed islet autoimmunity (n = 29) and autoantibody-negative control children (n = 29) with the HLA DR4-DQ8/DR3-DQ2 genotype were analyzed using two-dimensional gas chromatography coupled to a time-of-flight mass spectrometer for detection of small polar metabolites. Results: Plasma metabolite levels were found to depend strongly on age, with fold changes varying up to 50% from age 3 to 24 months (p < 0.001 after correction for multiple testing). Tyrosine levels tended to be lower in case children, but this was not significant after correction for multiple testing. Ornithine levels were lower in case children compared with the controls at the time of seroconversion, but the difference was not statistically significant after correcting for multiple testing. Breastfeeding for at least 3 months as compared with shorter duration was associated with higher plasma levels of isoleucine, and lower levels of methionine and 3,4-dihydroxybutyric acid at 3 months of age. Conclusions: Plasma levels of several small, polar metabolites changed with age during early childhood, independent of later islet autoimmunity status and sex. Breastfeeding was associated with higher levels of branched-chain amino acids, and lower levels of methionine and 3,4-dihydroxybutyric acid.
    No preview · Article · Jan 2016 · Pediatric Diabetes
  • Source
    • "In Europe, large outbreaks of polio-like disease caused by EV71 occurred in Bulgaria in 1975 and in Hungary in 1978. Thereafter, EV71 did not attract major attention until recently, when widespread asymptomatic circulation and detection from isolated neurological cases and minor outbreaks were reported from several European countries [5]–[8]. No major outbreaks have so far occurred in Europe. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Enterovirus 71 (EV71) is an emerging human pathogen causing massive epidemics of hand, foot and mouth disease with severe neurological complications in Asia. EV71 also circulates in Europe, however it does not cause large outbreaks. The reason for distinct epidemiological patterns of EV71 infection in Europe and Asia and the risk of EV71 epidemic in Europe and Russia remain unknown. Seroepidemiology of EV71 and molecular epidemiology of occasional EV71 isolates were studied to explore circulation of EV71 in Russia. In six regions of Russian Federation, seroprevalence of EV71 in sera collected in 2008 ranged from 5% to 20% in children aged 1-2 years and from 19% to 83% in children aged 3-5 years. The seroprevalence among elder children was significantly higher (41-83% vs. 19-27%) in Asian regions of Russia. EV71 strains identified in Russia in 2001-2011 belonged to subtypes C1 and C2, while genotype C4 that was causing epidemics in Asia since 1998 emerged in 2009 and became dominant in 2013.
    Full-text · Article · May 2014 · PLoS ONE
  • Source
    • "Han et al. reported positive rates of 0, 4.93 and 10.29% in throat swabs and 2.86, 3.94 and 8.82% in stools from three villages with different HFMD prevalence rate [9]. Studies conducted among children in Finland [33] and Norway [10,30,31] reported an EV71 positive rate of 0.3 and 1.4% from stool samples respectively, and isolation rate of 1.8% from stool samples were reported among children in Shenzhen, China [29]. Herein we demonstrated that 23.2% and 15.0% out of 254 healthy children carried HEV and EV71 respectively. "
    [Show abstract] [Hide abstract]
    ABSTRACT: To determine the prevalence of human enteroviruses (HEVs) among healthy children, their parents, and children with hand, foot, and mouth disease (HFMD). We conducted a case-control study that included throat samples from 579 children with HFMD and from 254 healthy controls. Throat samples from 49 households (98 parents and 53 healthy children) were also analyzed. Phylogenetic analysis was carried out to study genetic relationships of EV71 strains. The HEV positive rate in HFMD patients was significantly higher than that in healthy controls (76.0% vs. 23.2%, P < 0.001). The EV71 (43.7% vs. 15.0%, P < 0.001), CVA16 (18.0% vs. 2.8%, P < 0.001), and CVA10 (5.7% vs. 0.8%, P = 0.001) serotypes were significantly overrepresented in HFMD patients in comparison to healthy children. Other HEV serotypes were detected with comparable frequency in cases and controls. The HEV positive rate in severe HFMD patients was significantly higher than that in mild group (82.1% vs. 73.8%, P = 0.04). The EV71 (55.0% vs. 39.7%, P = 0.001) and CVA16 (11. 9% vs. 20.0%, P = 0.024) positive rate differed significantly between severe and mild HFMD patients. Other HEV serotypes were detected with comparable frequency between severe and mild HFMD patients. Among 49 households, 22 households (44.9%) had at least 1 family member positive for HEV. Children had significantly higher HEV positive rate than adult (28.3% vs. 14.3%, P = 0.037). The HEV positive rate was similar between mothers and fathers (12.24% vs. 16.32%, P = 0.56). The VP1 sequences of EV71 from HFMD patients and healthy children were nearly identical and all were clustered in the same clade, C4a. Our study demonstrated the co-circulation of multiple HEV serotypes in children with and without HFMD during epidemic. Our study deserves the attention on HFMD control.
    Full-text · Article · Dec 2013 · BMC Infectious Diseases
Show more