Poblet E, Jimenez F, Godinez JM et al.The immunohistochemical expression of CD34 in human hair follicles: a comparative study with the bulge marker CK15. Clin Exp Dermatol 31:807-812

University of Castilla-La Mancha, Ciudad Real, Castille-La Mancha, Spain
Clinical and Experimental Dermatology (Impact Factor: 1.09). 12/2006; 31(6):807-12. DOI: 10.1111/j.1365-2230.2006.02255.x
Source: PubMed


Anti-CD34 antibodies label the bulge region of mouse hair follicles. However, in human hair follicles, CD34 immunoreactivity is found in the outer root sheath below the bulge zone. The immunohistochemical staining of CD34 in catagen and telogen follicles has not been evaluated.
To characterize the expression of CD34 immunoreactivity at different stages of the hair cycle in human terminal hair follicles, and to compare the immunostaining pattern of CD34 with that of CK15, used here as a marker of the bulge region.
Serial vertical sections of human hair follicles in anagen, catagen and telogen phases were immunostained with anti-CD34 (QBEnd 10) and anti-CK15 (LHK15 and C8/144B) antibodies. Double-labelling immunofluorescence was also performed.
The catagen and telogen follicles studied did not show CD34 immunoreactivity in the outer root sheath. The location of CD34 and CK15 immunoreactivity in anagen follicles reveals a different staining pattern: CD34-positive cells are located in the outer root sheath below the attachment zone of the arrector pili muscle, whereas CK15-positive cells are located in the outer root sheath above the attachment zone of the arrector pili muscle.
Only anagen human hair follicles show CD34 immunoreactivity. CD34 and CK15 recognize different types of cells or cells at different stages of differentiation.

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    • "Although Lhx2 expression appears to be mandatory regarding the induction of hair growth from a quiescent telogen state, so far no specific epithelial subpopulation has been linked to it. In wild type, a CD49f expressing progenitor typically found in the basal layer of the epidermis [21], [22] is most predominant, followed by CD34 expressing progenitors that are most abundant at the root sheet level of hair follicles below the bulge region [23], [44], [45], [46], [47] (Fig. 1). In addition, a population highly (++) positive for CD49f can typically be distinguished from a population with intermediate (+) positivity. "
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    • "). Of note, CD34, a specific marker of mouse bulge stem cells, is underrepresented in human bulge cells (Ohyama et al., 2006; Poblet et al., 2006 ; Dog Human 1.6 1.2 0.8 0.4 0 LHX2 * * 0.3 0.2 0.1 0 LHX2 0.3 0.2 0.1 0 TCF3 0.06 0.04 0.02 0 TCF3 0.1 0.08 0.06 0.04 0.02 0 0.04 0.03 0 0.01 0.02 NFATC1 * 0.04 0.03 0 0.01 0.02 NFATC1 * "
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